Involvement of the late secretory pathway in actin regulation and mRNA transport in yeast
Involvement of the late secretory pathway in actin regulation and mRNA transport in yeast
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DOI：
10.1074/jbc.m402068200
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发表时间：
2004-08-27
影响因子：
4.8
通讯作者：
Gerst, JE
中科院分区：
文献类型：
--
作者：
Aronov, S;Gerst, JE
分泌囊泡的递送和mRNA向芽的不对称分布都依赖于酵母中的肌动蛋白细胞骨架。在这里，我们研究是否组成部分的胞外装置发挥作用的mRNA运输。通过筛选分泌突变体在原位和体内，我们发现，都有一个改变的模式ASH 1 mRNA定位。其中包括CDC 42和RHO 3的等位基因（CDC 42 -6和rho 3-V51），被认为可以特异性调节分泌囊泡的融合，但也被发现对细胞骨架有强烈影响。最有趣的是，不直接参与肌动蛋白调控的晚期分泌相关基因的突变也显示了ASH 1 mRNA分布的显著改变。这些包括编码外囊（SEC 10和SEC 15），SNARE调节蛋白（SEC 1，SEC 4和SRO 7），SNARE（SEC 9和SSO 1/2）和高尔基体输出相关蛋白（PIK 1和YPT 31/32）的基因突变。重要的是，在所有这些菌株中观察到肌动蛋白细胞骨架的显著缺陷，从而暗示了肌动蛋白失调和mRNA转运抑制之间的已知因果关系。我们的新的观察表明，囊泡运输调节肌动蛋白细胞骨架在酵母（而不仅仅是反之亦然），导致随后的缺陷，在mRNA的运输和定位。
Both the delivery of secretory vesicles and asymmetric distribution of mRNA to the bud are dependent upon the actin cytoskeleton in yeast. Here we examined whether components of the exocytic apparatus play a role in mRNA transport. By screening secretion mutants in situ and in vivo, we found that all had an altered pattern of ASH1 mRNA localization. These included alleles of CDC42 and RHO3 (cdc42-6 and rho3-V51) thought to regulate specifically the fusion of secretory vesicles but were found to affect strongly the cytoskeleton as well. Most interestingly, mutations in late secretion-related genes not directly involved in actin regulation also showed substantial alterations in ASH1 mRNA distribution. These included mutations in genes encoding components of the exocyst (SEC10 and SEC15), SNARE regulatory proteins (SEC1, SEC4, and SRO7), SNAREs (SEC9 and SSO1/2), and proteins involved in Golgi export (PIK1 and YPT31/32). Importantly, prominent defects in the actin cytoskeleton were observed in all of these strains, thus implicating a known causal relationship between the deregulation of actin and the inhibition of mRNA transport. Our novel observations suggest that vesicular transport regulates the actin cytoskeleton in yeast (and not just vice versa) leading to subsequent defects in mRNA transport and localization.