Fluorometric measurement of tin-protoporphyrin in biological samples.
Fluorometric measurement of tin-protoporphyrin in biological samples.
复制标题
生物样品中锡原卟啉的荧光测量。
DOI：
10.1016/0003-2697(84)90448-2
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发表时间：
1984
影响因子：
2.9
通讯作者：
Kappas,A
中科院分区：
文献类型：
--
作者：
Simionatto,CS;Anderson,KE;Sassa,S;Drummond,GS;Kappas,A
Sn-原卟啉是一种有效的血红素加氧酶的竞争性抑制剂，可以抑制实验室动物的新生儿和其他形式的高胆红素血症，并代表了一种潜在的新方法来治疗人类新生儿黄疸。为了研究Sn-原卟啉在体内的分布，我们建立了一种灵敏的荧光分析方法，用于生物样品中Sn-原卟啉的定量分析。该方法对低至0.01 nmol/ml的浓度敏感，并且即使在存在其他卟啉如原卟啉的情况下也对Sn-原卟啉具有特异性。
Sn-protoporphyrin is a potent competitive inhibitor of heme oxygenase, can suppress neonatal and other forms of hyperbilirubinemia in laboratory animals, and represents a potential new approach to the treatment of neonatal jaundice in humans. In order to study the disposition of Sn-protoporphyrin in vivo we have developed a sensitive fluorometric method for the quantitation of this metalloporphyrin in biological samples. The method is sensitive to concentrations as low as 0.01 nmol/ml, and is specific for Sn-protoporphyrin even in the presence of other porphyrins such as protoporphyrin.