BALB/c mice display more enhanced BCG vaccine induced Th1 and Th17 response than C57BL/6 mice but have equivalent protection
BALB/c mice display more enhanced BCG vaccine induced Th1 and Th17 response than C57BL/6 mice but have equivalent protection
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DOI：
10.1016/j.tube.2014.10.012
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发表时间：
2015-01-01
期刊：
影响因子：
3.2
通讯作者：
Hogarth, Philip J.
中科院分区：
文献类型：
--
作者：
Garcia-Pelayo, M. Carmen;Bachy, Veronique S.;Hogarth, Philip J.
人们普遍认为，近交系小鼠BALB/c（H - 2d）和C57BL/6（H - 2b）对分枝杆菌感染的反应中，T辅助细胞反应呈现明显的极化，C57BL/6倾向于Th1反应，而BALB/c倾向于Th2反应。我们在一个卡介苗免疫、牛分枝杆菌攻击模型中对此进行了研究。免疫后，测定了肺和脾细胞在体外用七种分泌型、免疫原性重组分枝杆菌蛋白混合物再次刺激后的细胞因子反应。在肺和脾中，BALB/c细胞产生的干扰素 - γ至少比C57BL/6细胞多2倍，白细胞介素 - 2和白细胞介素 - 17最多比C57BL/6细胞多7倍，而白细胞介素 - 10的产生在C57BL/6小鼠中则相应增加。这些数据表明，与文献报道相反，特定的分枝杆菌抗原在卡介苗接种后能够在BALB/c小鼠中诱导强烈的Th1和Th17反应，而在C57BL/6小鼠中，Th1反应部分被白细胞介素 - 10所抵消。在随后的低剂量牛分枝杆菌攻击后，通过对肺部和向脾脏扩散情况的检测，BALB/c和C57BL/6小鼠的保护作用相当，这表明卡介苗诱导的免疫在两种品系中是等效的。因此，不同的免疫反应似乎在保护作用中没有起到作用，而且进一步来说，还有尚未确定的特异性免疫反应起着重要作用。英国皇家版权所有（C）2014，由爱思唯尔有限公司出版。这是一篇在知识共享署名 - 非商业性使用 - 禁止演绎许可协议下的开放获取文章
It is generally assumed that the inbred mouse strains BALB/c (H-2(d)) and C57BL/6 ( H-2(b)) respond to mycobacterial infection with distinct polarisation of T helper responses, with C57BL/6 predisposed to Th1 and BALB/c to Th2. We investigated this in a BCG-immunisation, Mycobacterium bovis challenge model. Following immunisation, lung and spleen cell cytokine responses to in vitro re-stimulation with a cocktail of seven secreted, immunogenic, recombinant mycobacterial proteins were determined. In both lung and spleen, BALB/c cells produced at least 2-fold more IFN-gamma, and up to 7-fold more IL-2 and IL-17 than C57BL/6 cells, whereas IL-10 production was reciprocally increased in C57BL/6 mice. These data suggest that, contrary to reports in the literature, specific mycobacterial antigens are able to induce strong Th1 and Th17 responses in BALB/c mice following BCG vaccination, whilst in C57BL/6 mice, the Th1 response is partly counterbalanced by IL-10. After subsequent M. bovis low dose challenge, protection, as measured in the lungs and dissemination to the spleen, was equivalent in BALB/c and C57BL/6 mice, indicating that BCG-induced immunity was equivalent in both strains. Thus, the differential immune responses do not appear to have a role in protection, but further, as yet unidentified, specific immune responses play a significant role. Crown Copyright (C) 2014 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license