Spawning in the zebra mussel (Dreissena polymorpha): activation by internal or external application of serotonin.
Spawning in the zebra mussel (Dreissena polymorpha): activation by internal or external application of serotonin.
复制标题
斑马贻贝（Dreissena polymorpha）中产卵：通过内部或外部应用血清素激活。
DOI：
10.1002/jez.1402650515
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kyozuka,K
中科院分区：
文献类型：
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作者：
Ram,JL;Crawford,GW;Walker,JU;Mojares,JJ;Patel,N;Fong,PP;Kyozuka,K
斑马贻贝（Dreissena polymorpha）最近偶然被引入五大湖，部分由于其惊人的繁殖能力，在北美温带淡水中迅速蔓延。目前的研究考察了一些调节这种动物产卵的机制。在1990年8月和9月以及1991年5月，注射血清素（5‐羟色胺，5‐HT）诱导成熟雄性斑马贻贝产卵，而不是雌性斑马贻贝。在1991年仲夏期间，5‐HT诱导雄鱼和雌鱼产卵，5‐HT可以通过注射或外用产生产卵反应。外部pH值在较大范围内（6.0 - 9.1）对产卵没有影响，既不抑制5‐HT对产卵的诱导，也不显著地诱导产卵本身。外用10 - 3m和10 - 4m 5‐HT诱导产卵，但10 - 5m和10 - 6M没有。Cyproheptadine，一种5‐HT受体拮抗剂，使雄性和雌性的反应都降低了一半以上。5‐HT反应的产卵在4°C时被阻断，但在12°C、20°C或27°C时则没有。对于雄性斑马贻贝来说，判断性腺成熟度的形态学标准与5‐HT下的产卵概率密切相关。对于雌性来说，5‐HT对产卵的影响并不与性腺的形态成熟紧密相关，许多形态成熟的雌性不能产卵，一些明显不成熟的动物释放卵母细胞。先前的产卵降低了动物对5 - HT的后续反应性和产卵强度。这些实验支持5‐HT在调节斑马贻贝繁殖中的作用，并有助于确定刺激或抑制斑马贻贝产卵的条件。©1993 Wiley‐Liss, Inc。
The zebra mussel,Dreissena polymorpha, was recently introduced accidentally into the Great Lakes and, due in part to its prodigious reproductive capacity, is spreading rapidly in temperate fresh waters of North America. The present studies examine some of the mechanisms that regulate spawning in this animal.In August and September 1990 and in May 1991 injection of serotonin (5‐hydroxytryptamine, 5‐HT) induced ripe male, but not female, zebra mussels to spawn. During mid‐summer 1991, 5‐HT induced spawning in both males and females, and 5‐HT could produce spawning responses by either injection or external application. External pH over a broad range (6.0 to 9.1) had no effect on spawning, neither inhibiting induction of spawning by 5‐HT nor significantly eliciting spawning itself. With external application, 10–3M and 10–4M 5‐HT caused spawning, but 10–5M and 10−6M did not. Cyproheptadine, a 5‐HT receptor antagonist, reduced the response of both males and females by more than half. Spawning in response to 5‐HT was blocked at 4°C, but not at 12°C, 20°C, or 27°C. For male zebra mussels morphological criteria for judging gonadal maturity were well‐correlated with probability of spawning in response to 5‐HT. For females, the likelihood of spawning in response to 5‐HT was not tightly coupled to morphological maturity of the gonad, with many morphologically ripe females failing to spawn and some apparently immature animals releasing oocytes. Prior spawning reduced subsequent responsiveness and intensity of spawning of animals to 5‐HT. These experiments support a role for 5‐HT in regulating reproduction in zebra mussels and help define conditions by which zebra mussel spawning may be stimulated or inhibited. © 1993 Wiley‐Liss, Inc.