ROLE OF LYSOZYME IN MICROBICIDAL ACTIVITY OF RAT ALVEOLAR MACROPHAGES
ROLE OF LYSOZYME IN MICROBICIDAL ACTIVITY OF RAT ALVEOLAR MACROPHAGES
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DOI：
10.1128/iai.16.3.974-982.1977
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
STURGESS, JM
中科院分区：
文献类型：
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作者：
BIGGAR, WD;STURGESS, JM
溶菌酶从肺泡巨噬细胞的释放被暴露于颗粒如乳胶和酵母聚糖以及细菌刺激。大鼠肺泡巨噬细胞含有10倍以上的细胞内浓度的溶菌酶和释放更多的溶菌酶刺激后比大鼠血液中性粒细胞。在体外孵育30分钟期间，肺泡巨噬细胞杀死孵育混合物中超过99%的溶壁微球菌，而中性粒细胞杀死约50%的细菌。肺泡巨噬细胞对M.在所有测试的细菌与细胞比率下，溶壁细胞的比超过嗜中性粒细胞。当将特异性兔抗大鼠溶菌酶血清加入到孵育混合物中时，肺泡巨噬细胞的这种细菌杀伤作用被抑制。EM研究表明，细菌杀灭发生在细胞外。细菌的初始降解发生在5分钟内，裂解是由25 - 30分钟完成。裂解的细菌的吞噬作用是最大的25-30分钟后。更大量的溶菌酶，细胞内和释放到细胞外环境的肺泡巨噬细胞，这表明，这个因素可能是一种机制，肺泡巨噬细胞有助于肺防御。
Lysozyme release from alveolar macrophages is stimulated by exposure to particles, such as latex and zymosan, and to bacteria. Rat alveolar macrophages contain 10-fold greater intracellular concentrations of lysozyme and release more lysozyme after stimulation than rat blood neutrophils. During 30 min of incubation in vitro, alveolar macrophages kill more than 99% of Micrococcus lysodeikticus in the incubation mixture, whereas neutrophils kill approximately 50% of the bacteria. The bactericidal capacity of alveolar macrophages for M. lysodeikticus exceeds that of neutrophils at all bacteria-to-cell ratios tested. This bacterial killing by alveolar macrophages is inhibited when specific rabbit anti-rat lysozyme serum is added to the incubation mixture. EM studies indicate that bacterial killing occurs extracellularly. Inidial degradation of bacteria occurs within 5 min, and lysis is complete by 25-30 min. Phagocytosis of lysed bacteria is maximum after 25-30 min. The greater quantities of lysozyme, both intracellularly and released into the extracellular environment by alveolar macrophages, suggest that this factor may be a mechanism by which alveolar macrophages contribute to pulmonary defense.