DIVERSITY AND RELATIONSHIPS AMONG UNITED-STATES MAIZE INBREDS REVEALED BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS
DIVERSITY AND RELATIONSHIPS AMONG UNITED-STATES MAIZE INBREDS REVEALED BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS
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DOI：
10.2135/cropsci1991.0011183x003100030025x
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发表时间：
1991-05-01
期刊：
影响因子：
2.3
通讯作者：
LAMKEY, KR
中科院分区：
文献类型：
--
作者：
MELCHINGER, AE;MESSMER, MM;LAMKEY, KR
限制性片段长度多态性（RFLP）已被提议作为分子标记来表征玉米（Zea mays L.）的遗传多样性。本研究的目的是评估RFLP数据的有用性（i）阐明玉米自交系之间的杂种优势模式和（ii）评估相关和非相关系之间的遗传相似性。用分布在玉米基因组上的两种限制性内切酶和83个DNA探针分析了来自美国玉米带的32个玉米自交系的RFLP。82个探针检测到至少一种酶的多态性。平均而言，在所有32个近交系中，每个探针-酶组合发现4.3个变体。根据RFLP数据估计的品系间的遗传距离为罗杰斯距离（RD），揭示了来自爱荷华州硬茎合成（BSSS）、里德黄凹痕（赖德）和兰开斯特肯定作物（LSC）的品系间的相当大的多样性。来自不同的杂种优势组的线有一个略大的RD平均比无关的线从同一个杂种优势组，但差异很小，当与广泛的RD为每个组内的个别线组合。根据系谱数据确定的共祖系数，相关品系之间的RD与预期一致，只有少数例外。RFLP数据的主成分分析导致从BSSS/赖德和LSC线的单独分组。分散线的杂项来源一般是一致的预期的基础上已知的育种行为和谱系。从这项研究的结果表明，RFLP数据可用于分配到杂种优势群和相关线之间的遗传相似性量化的自交系，但似乎需要大量的探针-酶组合，以获得可靠的估计遗传距离。
Restriction fragment length polymorphisms (RFLPs) have been proposed as molecular markers for characterizing the genetic diversity in maize (Zea mays L.). The objectives of this study were to evaluate the usefulness of RFLP data for (i) elucidating heterotic patterns among maize inbreds and (ii) assessing genetic similarity among related and unrelated lines. Thirty-two maize inbreds from the U.S. Corn Belt were analyzed for RFLPs with two restriction enzymes and 83 DNA probes distributed over the maize genome. Eighty-two probes detected polymorphisms with at least one enzyme. On average, 4.3 variants were found per probe-enzyme combination across all 32 inbreds. Genetic distances among lines, estimated from RFLP data as Rogers' distance (RD), revealed considerable diversity among lines from Iowa Stiff Stalk Synthetic (BSSS), Reid Yellow Dent (RYD), and Lancaster Sure Crop (LSC). Lines from different heterotic groups had a slightly greater RD mean than unrelated lines from the same heterotic group, yet differences were small when compared with the wide range of RDs for individual lines combinations within each group. RDs between related lines agreed well with expectations based on coancestry coefficients determined from pedigree data with few exceptions. Principal component analyses of RFLP data resulted in a separate grouping of lines from BSSS/RYD and LSC. Dispersion of lines of miscellaneous origins was generally consistent with expectations based on known breeding behavior and pedigrees. Results from this study suggest that RFLP data can be used for assigning inbreds into heterotic groups and quantifying genetic similarity between related lines, but it seems that a large number of probe-enzyme combinations are required to obtain reliable estimates of genetic distance.