Invasive Infections Due to Trichosporon: Species Distribution, Genotyping, and Antifungal Susceptibilities from a Multicenter Study in China
Invasive Infections Due to Trichosporon: Species Distribution, Genotyping, and Antifungal Susceptibilities from a Multicenter Study in China
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毛孢子菌引起的侵袭性感染：中国多中心研究的物种分布、基因分型和抗真菌敏感性。
DOI：
10.1128/jcm.01505-18
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发表时间：
2019-02-01
影响因子：
9.4
通讯作者：
Xu, Ying-Chun
中科院分区：
文献类型：
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作者：
Guo, Li-Na;Yu, Shu-Ying;Xu, Ying-Chun
2009年至2016年，中国医院侵袭性真菌监测网（CHIF-NET）项目共收集了133株毛孢子菌临床分离株。通过基因间间隔区1（IGS 1）区域的测序进行准确鉴定。在这些分离物中，（108株（81.2%）为优势种，其次为皮氏丝孢酵母（7株[5.3%]），星状丝孢酵母（5株[3.8%]），毛孢子菌（5株分离株[3.8%]），多哈尔丝孢酵母（3株分离株[2.3%]）和1株分离株（0.7%）粪丝孢酵母、耶氏丝孢酵母、粘状丝孢酵母、玉米状丝孢酵母和蒙得维的亚丝孢酵母中的每一种。当物种存在于数据库中时，Vitek质谱（MS）（bioMerieux，Marcy l 'Etoile，France）和Bruker Biotyper MS（Bruker Daltonics GmbH，德国）平台均给出了高水平（>97.5%）的正确鉴定。对T. asahii是非asahii毛孢子菌的2倍。25%的T. 108株中有27株为阿沙希氏菌，25株中有4株为非阿沙希氏毛孢子菌，占16%。伊曲康唑MIC为。= 0.5 89.5%的分离株的抗真菌活性为μ g/ml。伏立康唑是体外最有效的抗真菌剂，GM为0.09 μ g/ml。IGS 1基因分型结果显示，基因型1最常见（41.7%），其次为基因型4（31.5%）、基因型3（23.1%）、基因型5（0.9%）、基因型6（0.9%）和基因型7（1.8%）。我们的物种分布，基因型和抗真菌药敏性的数据可能有助于更好地了解整个中国的侵袭性毛孢子菌感染的流行病学。
A total of 133 clinical Trichosporon isolates were collected in the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program in 2009 to 2016. Accurate identification was performed by sequencing of the intergenic spacer 1 (IGS1) region. Among these isolates, Trichosporon asahii (108 isolates (81.2%]) was the leading species, followed by Trichosporon dermatis (7 isolates [5.3%]), Trichosporon asteroides (5 isolates [3.8%]), Trichosporon inkin (5 isolates [3.8%]), Trichosporon dohaerlse (3 isolates [2.3%]), and 1 isolate (0.7%) each of Trichosporon faecale, Trichosporon jirovecii, Trichosporon mucoides, Trichosporon coremiiforme, and Trichosporon montevideense. Both the Vitek mass spectrometry (MS) (bioMerieux, Marcy l'Etoile, France) and Bruker Biotyper MS (Bruker Daltonics GmbH, Germany) platforms gave high levels (>97.5%) of correct identification when the species were present in the database. The geometric mean (GM) of amphotericin B MICs for T. asahii was 2-fold higher than that for non asahii Trichosporon. High fluconazole MICs (>= 8 mu g/ml) were observed for 25% of T. asahii isolates (27/108 isolates) and 16% of non asahii Trichosporon (4/25 isolates) isolates. Itraconazole MICs were .=0.5 mu g/ml for 89.5% of the isolates. Voriconazole was the most potent antifungal agent in vitro, with a GM of 0.09 mu g/ml. Genotyping of the isolates using IGS1 sequence alignment revealed that genotype 1 was most common (41.7%), followed by genotype 4 (31.5%), genotype 3 (23.1%), genotype 5 (0.9%), genotype 6 (0.9%), and genotype 7 (1.8%). Our data on species distribution, genotypes, and antifungal susceptibilities may contribute to a better understanding of the epidemiology of invasive Trichosporon infections throughout China.