The Influence of Dietary Protein Intake on Mammalian Tryptophan and Phenolic Metabolites.
The Influence of Dietary Protein Intake on Mammalian Tryptophan and Phenolic Metabolites.
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DOI：
10.1371/journal.pone.0140820
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Masereeuw R
中科院分区：
文献类型：
--
作者：
Poesen R;Mutsaers HA;Windey K;van den Broek PH;Verweij V;Augustijns P;Kuypers D;Jansen J;Evenepoel P;Verbeke K;Meijers B;Masereeuw R
尽管人们对高蛋白饮食的使用越来越感兴趣，但对哺乳动物代谢组中与饮食蛋白质相关的变化知之甚少。我们研究了蛋白质摄入对来自内源性和结肠微生物代谢的色氨酸和酚类化合物的影响。此外，还研究了潜在的物种间代谢差异。为此，29名健康受试者被分配到高蛋白饮食（n = 14）或低蛋白饮食（n = 15）两周。另外，将20只野生型FVB小鼠随机分为高蛋白组和对照组，为期21天。血浆和尿液样品采用液相色谱-质谱法测定色氨酸和酚类代谢物。在人类受试者中，我们观察到血浆中吲哚氧基硫酸酯水平和尿排泄量的显著变化（P 0.004和P 0.001），尿中吲哚氧基葡萄糖醛酸（P 0.01）、犬尿酸（P 0.006）和喹啉酸（P 0.02）的排泄量的显著变化。小鼠血浆色氨酸（P 0.03）、吲哚-3-乙酸（P 0.02）、对甲酰葡萄糖醛酸（P 0.03）、硫酸苯酯（P 0.004）和苯乙酸（P 0.01）含量差异显著。因此，膳食蛋白质摄入影响血浆水平和各种哺乳动物代谢物的产生，表明对内源性和结肠微生物代谢都有影响。代谢物的变化在人类受试者和小鼠之间是不同的，这表明物种间在蛋白质摄入方面的代谢差异。
Although there has been increasing interest in the use of high protein diets, little is known about dietary protein related changes in the mammalian metabolome. We investigated the influence of protein intake on selected tryptophan and phenolic compounds, derived from both endogenous and colonic microbial metabolism. Furthermore, potential inter-species metabolic differences were studied. For this purpose, 29 healthy subjects were allocated to a high (n = 14) or low protein diet (n = 15) for 2 weeks. In addition, 20 wild-type FVB mice were randomized to a high protein or control diet for 21 days. Plasma and urine samples were analyzed with liquid chromatography–mass spectrometry for measurement of tryptophan and phenolic metabolites. In human subjects, we observed significant changes in plasma level and urinary excretion of indoxyl sulfate (P 0.004 and P 0.001), and in urinary excretion of indoxyl glucuronide (P 0.01), kynurenic acid (P 0.006) and quinolinic acid (P 0.02). In mice, significant differences were noted in plasma tryptophan (P 0.03), indole-3-acetic acid (P 0.02), p-cresyl glucuronide (P 0.03), phenyl sulfate (P 0.004) and phenylacetic acid (P 0.01). Thus, dietary protein intake affects plasma levels and generation of various mammalian metabolites, suggesting an influence on both endogenous and colonic microbial metabolism. Metabolite changes are dissimilar between human subjects and mice, pointing to inter-species metabolic differences with respect to protein intake.