Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model.
Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model.
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转录组分析揭示彭德雷综合征小鼠模型血管纹中同型半胱氨酸代谢的改变
DOI：
10.1155/2021/5585394
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发表时间：
2021
影响因子：
3.1
通讯作者：
Yin S
中科院分区：
文献类型：
--
作者：
Xue W;Tian Y;Xiong Y;Liu F;Feng Y;Chen Z;Yu D;Yin S
Slc26a4−/− mice exhibit severer defects in the development of the cochlea and develop deafness, while the underlying mechanisms responsible for these effects remain unclear. Our study was to investigate the potential mechanism linking SLC26A4 deficiency to hearing loss. RNA sequencing was applied to analyze the differential gene expression of the stria vascularis (SV) from wildtype and Slc26a4−/− mice. GO and KEGG pathway analysis were performed. Quantitative RT-PCR was applied to validate the expression of candidate genes affected by Slc26a4. ELISA and immunofluorescence technique were used to detect the homocysteine (Hcy) level in serum, brain, and SV, respectively. 183 upregulated genes and 63 downregulated genes were identified in the SV associated with Slc26a4 depletion. Transcriptomic profiling revealed that Slc26a4 deficiency significantly affected the expression of genes associated with cell adhesion, transmembrane transport, and the biogenesis of multicellular organisms. The SV from Slc26a4−/− mice exhibited a higher expression of Bhmt mRNAs, as well as altered homocysteine (Hcy) metabolism. The altered expression of Bhmt results in a dramatic change in multiple biochemical reactions and a disruption of nutrient homeostasis in the endolymph which may contribute to hearing loss of Slc26a4 knockout mouse.
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影响因子：
5.6
作者：
Martinez-Vega, Raquel;Partearroyo, Teresa;Varela-Nieto, Isabel
通讯作者：
Varela-Nieto, Isabel
Wnt 激活可防止新霉素诱导的小鼠耳蜗毛细胞损伤
影响因子：
9
作者：
Liu L;Chen Y;Qi J;Zhang Y;He Y;Ni W;Li W;Zhang S;Sun S;Taketo MM;Wang L;Chai R;Li H
通讯作者：
Li H
影响因子：
9.3
作者：
Jabba SV;Oelke A;Singh R;Maganti RJ;Fleming S;Wall SM;Everett LA;Green ED;Wangemann P
通讯作者：
Wangemann P
影响因子：
3.7
作者：
Kim HM;Wangemann P
通讯作者：
Wangemann P
影响因子：
5.5
作者：
Marcus, DC;Wu, T;Kofuji, P
通讯作者：
Kofuji, P