Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica

Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica
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DOI:
10.1007/978-1-4939-6927-2_6
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发表时间:
2017-01-01
期刊:
BACTERIAL FLAGELLUM: METHODS AND PROTOCOLS
影响因子:
--
通讯作者:
Aizawa, Shin-Ichi
Aizawa, Shin-Ichi
中科院分区:
其他
文献类型:
--
作者:
Aizawa, Shin-Ichi

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The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I describe protocols for the purification of the filament and hook-basal body from Salmonella enterica serovar Typhimurium.