Transplacental transfer of monomethyl phthalate and mono( 2-ethylhexyl) phthalate in a human placenta perfusion system
Transplacental transfer of monomethyl phthalate and mono( 2-ethylhexyl) phthalate in a human placenta perfusion system
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DOI：
10.1080/10915810701352721
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Mortensen, Gerda K.
中科院分区：
文献类型：
--
作者：
Mose, Tina;Knudsen, Lisbeth E.;Mortensen, Gerda K.
采用体外胎盘灌注模型，在单子叶内同时灌注胎儿和母体循环，研究了邻苯二甲酸一甲酯（mMP）和邻苯二甲酸一（2-乙基己基）酯（mEHP）经胎盘的传代。并对灌注前后采集的脐带血和胎盘组织进行分析。择期剖宫产术后立即取胎盘，在循环系统中双灌注。在母体灌注培养基中加入mMP或mEHP，浓度分别为10和25 μ g/L。采用液相色谱-质谱联用-质谱联用（LC-MS-MS）对胎儿和母体灌注培养基样品进行胎盘移植观察。4次灌注mMP提示胎盘转移缓慢，灌注150 min后胎母比（FM）为0.30 +/- 0.03。四次mEHP灌注表明胎盘转移非常缓慢或不存在。mEHP仅在两次灌注的胎儿灌注介质中检测到，灌注150 min后FM比分别为0.088和0.20。在组织中发现了可检测水平的mMP， mEHP，邻苯二甲酸一乙酯（mEP）和邻苯二甲酸一丁酯。与mEHP灌注相比，mMP灌注后mMP的组织水平较高，表明灌注过程中mMP的积累。与mMP灌注相比，mEHP灌注期间未观察到mEHP积累的趋势。脐带血浆样品中检测到mEHP和mEP的水平。母体血液中的mMP和可能的其他短链邻苯二甲酸酯单酯可以通过缓慢的转移穿过胎盘，而结果表明mEHP没有胎盘转移。建议进一步研究。
The transplacental passage of monomethylphtalate ( mMP) and mono (2-ethylhexyl) phthalate (mEHP) was studied using an ex vivo placental perfusion model with simultaneous perfusion of fetal and maternal circulation in a single cotyledon. Umbilical cord blood and placental tissue collected both before and after perfusion were also analyzed. Placentas were obtained immediately after elective cesarean section and dually perfused in a recirculation system. mMP or mEHP was added to maternal perfusion medium to obtain concentrations at 10 and 25 mu g/L, respectively. The placental transfer was followed analyzing samples from fetal and maternal perfusion media by liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). Four perfusions with mMP indicated a slow transplacental transfer, with a feto-maternal ratio ( FM ratio) of 0.30 +/- 0.03 after 150 min of perfusion. Four perfusions with mEHP indicated a very slow or nonexisting placental transfer. mEHP was only detected in fetal perfusion media from two perfusions, giving rise to FM ratios of 0.088 and 0.20 after 150 min of perfusion. Detectable levels of mMP, mEHP, monoethylphthalate (mEP), and monobutylphthalate were found in tissue. Higher tissue levels of mMP after perfusions with mMP compared to perfusions with mEHP suggest an accumulation of mMP during perfusion. No tendency for accumulation of mEHP was observed during perfusions with mEHP compared to perfusions with mMP. Detectable levels of mEHP and mEP were found in umbilical cord plasma samples. mMP and possibly other short-chained phthalate monoesters in maternal blood can cross the placenta by slow transfer, whereas the results indicate no placental transfer of mEHP. Further studies are recommended.