MicroRNA-target gene responses to lead-induced stress in cotton (Gossypium hirsutum L.)
MicroRNA-target gene responses to lead-induced stress in cotton (Gossypium hirsutum L.)
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DOI：
10.1007/s10142-014-0378-z
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发表时间：
2014-09-01
影响因子：
2.9
通讯作者：
Zhang, Baohong
中科院分区：
文献类型：
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作者：
He, Qiuling;Zhu, Shuijin;Zhang, Baohong
MicroRNAs （miRNAs）在植物对各种金属胁迫的反应中起着关键作用。为了研究mirna介导的植物对重金属的响应，以棉花（Gossypium hirsutum L.）为研究对象，研究了不同浓度（0、25、50、100和200 μ M）铅对棉花的毒理学效应。采用实时荧光定量PCR （qRT-PCR）技术，对棉花叶片和根系中16个胁迫应答mirna和10个靶基因的表达谱进行了监测；在这些被选中的基因中，有几个mirna及其靶基因参与了根的发育。结果表明，mirna在棉花对铅胁迫的响应中具有相互调节作用。研究这些miRNA和相关靶基因对铅暴露的响应将有助于确定miRNA在植物重金属胁迫适应中的潜在作用，并进一步了解miRNA在非生物胁迫响应中的调控作用。
MicroRNAs (miRNAs) play key roles in plant responses to various metal stresses. To investigate the miRNA-mediated plant response to heavy metals, cotton (Gossypium hirsutum L.), the most important fiber crop in the world, was exposed to different concentrations (0, 25, 50, 100, and 200 A mu M) of lead (Pb) and then the toxicological effects were investigated. The expression patterns of 16 stress-responsive miRNAs and 10 target genes were monitored in cotton leaves and roots by quantitative real-time PCR (qRT-PCR); of these selected genes, several miRNAs and their target genes are involved in root development. The results show a reciprocal regulation of cotton response to lead stress by miRNAs. The characterization of the miRNAs and the associated target genes in response to lead exposure would help in defining the potential roles of miRNAs in plant adaptation to heavy metal stress and further understanding miRNA regulation in response to abiotic stress.