Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors
Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors
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DOI：
10.1073/pnas.0508214103
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发表时间：
2006-03-07
影响因子：
11.1
通讯作者：
Swaroop, A
中科院分区：
文献类型：
--
作者：
Akimoto, M;Cheng, H;Swaroop, A
Maf家族转录因子NRL是哺乳动物光感受器分化的关键调控因子。去除小鼠的NRL基因导致了功能锥体的产生，但损害了杆状细胞。我们发现，2.5kb的NRL启动子片段将增强的GFP特异性地导向杆状感光细胞和转基因小鼠的松果体。绿色荧光蛋白是在末端细胞分裂后不久检测到的，与出生年龄研究揭示的杆状细胞发生的时间相对应。在NRL(-/-)视网膜，绿色荧光蛋白阳性的光感受器表达S视蛋白，这与视杆前体细胞向视锥细胞的转化相一致。我们报道了新鲜分离的来自野生型和NRL(-/-)视网膜的流动分离的GFP+光感受器在五个不同的发育阶段的基因图谱。我们的结果为建立基因调控网络提供了一个框架，该网络可以从有丝分裂后的前体细胞产生成熟的功能性光感受器。差异表达的视杆细胞和视锥细胞基因是视网膜病变的极佳候选基因。
The Maf-family transcription factor Nrl is a key regulator of photoreceptor differentiation in mammals. Ablation of the Nrl gene in mice leads to functional cones at the expense of rods. We show that a 2.5-kb Nrl promoter segment directs the expression of enhanced GFP specifically to rod photoreceptors and the pineal gland of transgenic mice. GFP is detected shortly after terminal cell division, corresponding to the timing of rod genesis revealed by birthdating studies. In Nrl(-/-) retinas, the GFP+ photoreceptors express S-opsin, consistent with the transformation of rod precursors into cones. We report the gene profiles of freshly isolated flow-sorted GFP+ photoreceptors from wild-type and Nrl(-/-) retinas at five distinct developmental stages. Our results provide a framework for establishing gene regulatory networks that lead to mature functional photoreceptors from postmitotic precursors. Differentially expressed rod and cone genes are excellent candidates for retinopathies.