A new activity of complement component C3: cell-bound C3b potentiates lysis of erythrocytes by C5b,6 and terminal components.
A new activity of complement component C3: cell-bound C3b potentiates lysis of erythrocytes by C5b,6 and terminal components.
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补体成分 C3 的新活性：细胞结合的 C3b 增强 C5b,6 和末端成分对红细胞的裂解。
DOI：
10.4049/jimmunol.117.3.830
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发表时间：
1976
影响因子：
4.4
通讯作者：
M. Mayer
中科院分区：
文献类型：
--
作者：
C. Hammer;A. Abramovitz;M. Mayer
EAC4b,3b（羊红细胞携带兔抗体和豚鼠补体组分片段C4b和C3）对人C5b，6的吸附是可逆的；结合的快速性与离子强度成反比。我们认为C5b，6的受体是由细胞结合的C3b贡献的，因为EAC4b，3b的结合能力随C3b的多样性而变化，并且可以被兔抗豚鼠C3抗体阻断。C5b，6与红细胞结合的C3b的固定作用是将C5b，6集中在细胞表面；因此，用EAC4b，3b测定C5b，6的溶血效率几乎是用普通红细胞测定的100倍。这种增强代表了迄今为止未被认识的细胞结合C3b的功能。
EAC4b,3b (sheep erythrocytes carrying rabbit antibody and guinea pig complement component fragments C4b and C3) adsorb human C5b,6 reversibly; the avidity of binding varies inversely with ionic strength. We believe that the receptor of C5b,6 is contributed by the cell-bound C3b because the binding capacity of EAC4b,3b varies with C3b multiplicity and can be blocked with rabbit antibody to guinea pig C3. The fixation of C5b,6 to the erythrocyte-bound C3b serves to concentrate C5b,6 on the cell surface; as a consequence, the hemolytic efficiency of C5b,6 is almost 100 times greater when assayed with EAC4b,3b than with plain erythrocytes. This potentiation represents a hitherto unrecognized function of cell-bound C3b.