Identifying the essential nutritional requirements of the probiotic bacteria Bifidobacterium animalis and Bifidobacterium longum through genome-scale modeling.
Identifying the essential nutritional requirements of the probiotic bacteria Bifidobacterium animalis and Bifidobacterium longum through genome-scale modeling.
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DOI：
10.1038/s41540-021-00207-4
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发表时间：
2021-12-09
影响因子：
4
通讯作者：
Zeidan AA
中科院分区：
文献类型：
--
作者：
Schöpping M;Gaspar P;Neves AR;Franzén CJ;Zeidan AA
Although bifidobacteria are widely used as probiotics, their metabolism and physiology remain to be explored in depth. In this work, strain-specific genome-scale metabolic models were developed for two industrially and clinically relevant bifidobacteria, Bifidobacterium animalis subsp. lactis BB-12® and B. longum subsp. longum BB-46, and subjected to iterative cycles of manual curation and experimental validation. A constraint-based modeling framework was used to probe the metabolic landscape of the strains and identify their essential nutritional requirements. Both strains showed an absolute requirement for pantethine as a precursor for coenzyme A biosynthesis. Menaquinone-4 was found to be essential only for BB-46 growth, whereas nicotinic acid was only required by BB-12®. The model-generated insights were used to formulate a chemically defined medium that supports the growth of both strains to the same extent as a complex culture medium. Carbohydrate utilization profiles predicted by the models were experimentally validated. Furthermore, model predictions were quantitatively validated in the newly formulated medium in lab-scale batch fermentations. The models and the formulated medium represent valuable tools to further explore the metabolism and physiology of the two species, investigate the mechanisms underlying their health-promoting effects and guide the optimization of their industrial production processes.
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影响因子：
5.4
作者：
Feist, Adam M.;Palsson, Bernhard O.
通讯作者：
Palsson, Bernhard O.
DOI：
10.1080/00021369.1985.10866683
发表时间：
1985-01-01
期刊：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY
影响因子：
--
作者：
DEGUCHI, Y;MORISHITA, T;MUTAI, M
通讯作者：
MUTAI, M
影响因子：
5
作者：
DEGNAN, BA;MACFARLANE, GT
通讯作者：
MACFARLANE, GT
影响因子：
--
作者：
El-Semman IE;Karlsson FH;Shoaie S;Nookaew I;Soliman TH;Nielsen J
通讯作者：
Nielsen J
影响因子：
4.3
作者：
Feist AM;Nagarajan H;Rotaru AE;Tremblay PL;Zhang T;Nevin KP;Lovley DR;Zengler K
通讯作者：
Zengler K