Sequence analysis of a cDNA coding for a pancreatic precursor to somatostatin.
Sequence analysis of a cDNA coding for a pancreatic precursor to somatostatin.
复制标题
编码胰腺生长抑素前体的 cDNA 的序列分析。
DOI：
10.1073/pnas.78.11.6694
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Dixon,JE
中科院分区：
文献类型：
--
作者：
Taylor,WL;Collier,KJ;Deschenes,RJ;Weith,HL;Dixon,JE
具有从生长抑素-14的氨基酸序列Phe-Phe-Trp-Lys推导的序列d（T-T-C-C-A-G-A-A-G-A-A）的合成寡核苷酸用于引发从斑点叉尾鱼（Ictalurus punctatus）胰腺多聚（A）-RNA合成cDNA。该反应的主要产物是565个核苷酸的cDNA片段。cDNA片段的化学序列分析表明，它是互补的生长抑素的mRNA编码。565个核苷酸的cDNA与估计长度为1000个核苷酸的聚（A）-RNA强烈杂交。从核苷酸序列预测生长抑素前体的氨基酸序列。Oyama等人[Oyama，H.，布拉德肖河一、贝茨岛J. & Permutt，A.（1980）J.Biol.Chem.255，2251-2254]报道了从鲶鱼中分离出长度为22个残基的生长抑素（生长抑素-22）。该肽在氨基酸序列上不同于生长抑素-14。本实验室获得的cDNA序列编码生长抑素-14，并预测来自该物种的另一种生长抑素基因产物。因此，似乎至少有两种生长抑素基因产物。
A synthetic oligonucleotide having the sequence d(T-T-C-C-A-G-A-A-G-A-A) deduced from the amino acid sequence Phe-Phe-Trp-Lys of somatostatin-14 was used to prime the synthesis of a cDNA from channel catfish (Ictalurus punctatus) pancreatic poly(A)-RNA. The major product of this reaction was a cDNA fragment of 565 nucleotides. Chemical sequence analysis of the cDNA fragment revealed that it was complementary to a mRNA coding for somatostatin. The 565-nucleotide cDNA hybridizes strongly with a poly(A)-RNA estimated to be 1000 nucleotides in length. An amino acid sequence of the somatostatin precursor was predicted from the nucleotide sequence. Oyama et al. [Oyama, H., Bradshaw, R. A., Bates, O. J. & Permutt, A. (1980) J. Biol. Chem. 255, 2251-2254] have reported the isolation of a somatostatin from the catfish that is 22 residues in length (somatostatin-22). This peptide differs from somatostatin-14 in amino acid sequence. The cDNA sequence obtained by this laboratory codes for somatostatin-14 and predicts another somatostatin gene product from this species. Thus it would appear that there are at least two somatostatin gene products.