Microcosm Biofilms Originating from Children with Different Caries Experience Have Similar Cariogenicity under Successive Sucrose Challenges
Microcosm Biofilms Originating from Children with Different Caries Experience Have Similar Cariogenicity under Successive Sucrose Challenges
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DOI：
10.1159/000331210
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发表时间：
2011-01-01
期刊：
影响因子：
4.2
通讯作者：
Cenci, M. S.
中科院分区：
文献类型：
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作者：
Azevedo, M. S.;van de Sande, F. H.;Cenci, M. S.
目的：唾液接种来源和唾液供者的个体特征可以影响体外生物膜的致龋性，但这也可以受到环境决定因素的调节，如糖的消耗频率。因此，本研究的目的是比较常规蔗糖暴露下无龋组(CF)、早期龋组(ECC)和重度组(S-ECC)儿童唾液微宇宙生物膜生长的致龋性。方法：从CF、ECC和S-ECC儿童的唾液中建立微宇宙菌斑生物膜。生物膜生长在牛牙釉质圆盘上的24孔微孔板中，在人工唾液中培养长达10天，每天更换。生长条件包括致龋性挑战(人工唾液中添加1%蔗糖，每天6h)或无致龋性挑战。从人工唾液中获取每日pH值，实验结束后，收集釉质盘上形成的生物膜进行微生物分析。釉质盘中矿物质的损失是通过表面硬度变化的百分比来估计的。结果：总体而言，唾液来源之间没有统计学上的显著差异(p>；0.05)。在致龋性攻击下生长的生物膜中变形链球菌和乳杆菌数量增加(p<；0.001)，而人工唾液pH在相同条件下显著降低(p<；0.05)。无论儿童有无龋齿经历，在蔗糖暴露下观察到更高的脱矿率(p<；0.001)。结论：虽然蔗糖暴露决定了生物膜的致龋性，但提供接种的儿童的龋病经历并不影响与这些生物膜相关的矿物质损失。版权所有(C)2011 S.Karger AG，
Objectives: The source of saliva inocula and the individual characteristics of saliva donors could affect the cariogenic activity of in vitro biofilms, but this could also be modulated by environmental determinants, such as the frequency of sugar consumption. Therefore, the aim of this study was to compare the cariogenicity of microcosm biofilm growths from the saliva of caries-free (CF) children, children with early childhood caries (ECC) and with severe ECC (S-ECC), under regular sucrose exposure. Methods: Microcosm plaque biofilms were initiated from the saliva of CF, ECC and S-ECC children. Biofilms were grown in 24-well microplates on bovine enamel discs for up to 10 days in artificial saliva, which was replaced daily. Growth conditions comprised cariogenic challenge (artificial saliva supplemented with 1% sucrose 6 h/day) or no cariogenic challenge. Daily pH was obtained from the artificial saliva, and after the experimental period, the biofilm formed on the enamel discs was collected for microbiological analyses. Mineral loss in enamel discs was estimated by percentage of surface hardness change. Results: Overall, no statistically significant differences were found among saliva sources (p > 0.05). Streptococcus mutans and lactobacilli counts increased in the biofilms grown under cariogenic challenge (p < 0.05), while a substantial decrease in the artificial saliva pH was detected under the same condition (p < 0.001). Higher demineralization (p < 0.001) was observed under sucrose exposure regardless of caries experience of children. Conclusions: While the sucrose exposure determined the cariogenicity of the biofilms, the caries experience of children who provided the inocula did not affect mineral loss associated with these biofilms. Copyright (C) 2011 S. Karger AG,