Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography.
Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography.
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DOI：
10.1016/j.carbpol.2013.07.011
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发表时间：
2013-10
影响因子：
11.2
通讯作者：
Jianhua Xie;Mingyue Shen;Shaoping Nie;Xin Liu;Hui Zhang;M. Xie
中科院分区：
文献类型：
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作者：
Jianhua Xie;Mingyue Shen;Shaoping Nie;Xin Liu;Hui Zhang;M. Xie
建立了一种简单、快速、灵敏的测定白环多糖中单糖成分的分析方法，并进行了验证。该方法采用高效阴离子交换色谱法对多糖进行水解。采用Carbo PAC™PA10分析柱研究氢氧化钠浓度和柱温对单糖保留和分离的影响。结果表明，该方法具有良好的线性关系（R2为0.9987 ~ 0.9999）、良好的准确度（回收率为98.53 ~ 102.13%）、较高的精密度（相对标准偏差<3.8%）和灵敏的检出限（2.57 ~ 7.86 nM）、制备样品简单（无需衍生）、运行时间短（20 min）等优点。结果表明，c。鼠李糖多糖由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖组成，摩尔比为1.00:1.85:3.26:3.12:0.85:0.29。
A simple, rapid and sensitive analytical method for the determination of monosaccharide composition inCyclocarya paliuruspolysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC™ PA10 analytical column. The established method was validated and the results showed that this method had good linearity (R2, 0.9987–0.9999), adequate accuracy (98.53–102.13% recovery), high precision (relative standard deviation <3.8%) and sensitive detection limits (2.57–7.86 nM), with a simple preparation of the samples (no need to derivatize the samples) and short run time (20 min). The results showed that theC. paliuruspolysaccharide consisted of rhamnose, arabinose, galactose, glucose, mannose and xylose in the molar ratio of 1.00:1.85:3.26:3.12:0.85:0.29.