Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo
Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo
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DOI：
10.1016/s0006-291x(02)02013-2
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发表时间：
2002-08-30
影响因子：
3.1
通讯作者：
Malvy, C
中科院分区：
文献类型：
--
作者：
Bertrand, JR;Pottier, M;Malvy, C
在细胞培养和异种移植小鼠中比较了针对HeLa细胞中稳定表达的绿色荧光蛋白的核酸酶抗性反义寡核苷酸和siRNA的效率。使用细胞素GSV递送这两种抑制物，siRNAs似乎在数量上更有效，其作用在细胞培养中持续更长时间。在小鼠身上，我们观察到了siRNA的活性，但没有观察到反义寡核苷酸的活性。反义寡核苷酸缺乏效率可能是因为它们对核酸酶降解的抵抗力较低。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Efficiencies of a nuclease resistant antisense oligonucleotide and of siRNA both being targeted against the green fluorescent protein stably expressed in HeLa cells are compared in cell cultures and in xenografted mice. Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture. In mice, we observed an activity of siRNAs but not of antisense oligonucleotides. The absence of efficiency of antisense oligonucleotides is probably due to their lower resistance to nuclease degradation. (C) 2002 Elsevier Science (USA). All rights reserved.