Surface characteristics of the microbial cell of Pseudomonas syringae and its relevance to cell attachment
Surface characteristics of the microbial cell of Pseudomonas syringae and its relevance to cell attachment
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丁香假单胞菌微生物细胞的表面特征及其与细胞附着的相关性
DOI：
10.1016/s0927-7765(97)00023-4
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Morisaki
中科院分区：
文献类型：
--
作者：
S. Takashima;H. Morisaki
4株细菌的细胞表面均为假单胞菌pv. atropurpurea NIAES 1309通过分析微生物细胞的电泳迁移率对pH和离子强度的依赖性来表征。在这些菌株中，野生型（WT）具有最少的电荷，同时在表面携带最软的聚合物层。通过化学分析确定WT菌株的细胞表面富含糖，因此由于水合作用而显得庞大。WT菌株贴附在玻璃板上的细胞数量最少。这些发现导致了这样的假设，即细胞表面的柔软且水合的聚合物层可能会阻碍细胞附着。
The cell surface of four strains of Pseudomonas syringae pv. atropurpurea NIAES 1309 was characterized by analyzing the dependence of the electrophoretic mobility of the microbial cells on pH and ionic strength. Among the strains, the wild type (WT) had the least electric charge, while carrying the softest polymer layer, at the surface. The cell surface of the WT strain was determined, by chemical analysis, to be rich in sugar, and thus seems to be bulky because of hydration. The number of cells attached to a glass plate was least for the WT strain. These findings led to the assumption that a soft and hydrated polymer layer at the cell surface may hinder the cell attachment.