Wavelength dependence of crosstalk in dual-wavelength measurement of oxy- and deoxy-hemoglobin
Wavelength dependence of crosstalk in dual-wavelength measurement of oxy- and deoxy-hemoglobin
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DOI：
10.1117/1.1846076
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发表时间：
2005-01-01
影响因子：
3.5
通讯作者：
Okada, E
中科院分区：
文献类型：
--
作者：
Okui, N;Okada, E
在近红外光谱中，组织中氧合血红蛋白和脱氧血红蛋白的浓度变化是通过求解基于修正的朗伯-比尔定律的联立方程，从两个波长处检测到的光强度的变化来计算的。波长无关常数或平均光程长通常被指定为联立方程中的部分光程长项。在计算中，这种不足的光程长度导致氧合血红蛋白和脱氧血红蛋白的浓度变化之间的串扰。通过蒙特卡罗模拟，对氧合血红蛋白和脱氧血红蛋白双波长测量中的串扰进行了理论研究，讨论了减小串扰的最佳波长对。双波长测量的较长波长固定在830 nm，较短波长在650至780 nm之间变化。与830 nm配对用于氧合血红蛋白和脱氧血红蛋白双波长测量的最佳波长范围为690至750 nm。通过时间分辨和相位分辨测量得到的平均光程长度是减小双波长测量结果中串扰的有效方法。(c)2005年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
In near-IR spectroscopy, the concentration change in oxy- and deoxyhemoglobin in tissue is calculated from the change in the detected intensity of light at two wavelengths by solving the simultaneous equation based on the modified Lambert-Beer law. The wavelength-independent constant or mean optical path length is usually assigned to the term of partial optical path length in the simultaneous equation. This insufficient optical path length in the calculation causes crosstalk between the concentration change in oxy- and deoxyhemoglobin. We investigate the crosstalk in the dual-wavelength measurement of oxy- and deoxyhemoglobin theoretically by Monte Carlo simulation to discuss the optimal wavelength pair to minimize the crosstalk. The longer wavelength of the dual-wavelength measurement is fixed at 830 nm and the shorter wavelength is varied from 650 to 780 nm. The optimal wavelength range for pairing with 830 nm for the dual-wavelength measurement of oxy- and deoxyhemoglobin is from 690 to 750 nm. The mean optical path length, which can be obtained by time- and phase-resolved measurement, is effective to reduce the crosstalk in the results of dual-wavelength measurement. (c) 2005 Society of Photo-Optical Instrumentation Engineers.