FgBUD14 is important for ascosporogenesis and involves both stage-specific alternative splicing and RNA editing during sexual reproduction
FgBUD14 is important for ascosporogenesis and involves both stage-specific alternative splicing and RNA editing during sexual reproduction
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FgBUD14 对于子囊孢子发生很重要，涉及有性生殖过程中阶段特异性的选择性剪接和 RNA 编辑
DOI：
10.1111/1462-2920.15446
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发表时间：
2021
影响因子：
5.1
通讯作者：
Wang Guanghui
中科院分区：
文献类型：
--
作者：
Liang Jie;Fu Xianhui;Hao Chaofeng;Bian Zhuyun;Liu Huiquan;Xu Jin-Rong;Wang Guanghui
在小麦赤霉病真菌禾谷镰刀菌中，A-to-I RNA编辑特异性地发生在有性生殖期间。在具有需要RNA编辑以编码全长蛋白质的过早终止密码子（PSC）的基因中，FgBUD 14在包皮中也具有选择性剪接事件。在这项研究中，我们的特点FgBUD 14的功能和转录后修饰在有性生殖过程中。Fgbud 14缺失突变体在生长、产孢和毒力方面略有降低。虽然FgBUD 14的缺失对子囊壳形态没有影响，但FgBUD 14突变体在克罗泽形成和发育中有缺陷。FgBud 14-GFP主要定位于子囊菌丝顶端和茎节，可能与其在性发育早期的功能有关。在营养生长和无性繁殖过程中，FgBud 14-GFP定位于菌丝顶端和分生孢子两端。此外，阻断具有PSC位点的内含子2的剪接的突变对FgBUD 14在有性生殖期间的功能没有影响，但对FgBUD 14突变体的生长造成类似的缺陷。非可编辑的FgBUD 14内含子2-TAA突变体等位基因的表达也不能补充FgbuD 14突变体。总之，FgBUD 14在果蝇发育中起着重要作用，并且在果蝇有性生殖过程中，选择性剪接和RNA编辑都特异性地发生在其转录本上。禾谷早熟禾
In wheat head blight fungus Fusarium graminearum, A-to-I RNA editing occurs specifically during sexual reproduction. Among the genes with premature stop codons (PSCs) that require RNA editing to encode full-length proteins, FgBUD14 also had alternative splicing events in perithecia. In this study, we characterized the functions of FgBUD14 and its post-transcriptional modifications during sexual reproduction. The Fgbud14 deletion mutant was slightly reduced in growth, conidiation and virulence. Although deletion of FgBUD14 had no effect on perithecium morphology, the Fgbud14 mutant was defective in crozier formation and ascus development. The FgBud14-GFP localized to the apex of ascogenous hyphae and croziers, which may be related to its functions during early sexual development. During vegetative growth and asexual reproduction, FgBud14-GFP localized to hyphal tips and both ends of conidia. Furthermore, mutations blocking the splicing of intron 2 that has the PSC site had no effect on the function of FgBUD14 during sexual reproduction but caused a similar defect in growth with Fgbud14 mutant. Expression of the non-editable FgBUD14Intron2-TAA mutant allele also failed to complement the Fgbud14 mutant. Taken together, FgBUD14 plays important roles in ascus development, and both alternative splicing and RNA editing occur specifically to its transcripts during sexual reproduction in F. graminearum.