Activation of mouse macrophages for tumor cell killing. I. Quantitative analysis of interactions between lymphokine and lipopolysaccharide.
Activation of mouse macrophages for tumor cell killing. I. Quantitative analysis of interactions between lymphokine and lipopolysaccharide.
复制标题
激活小鼠巨噬细胞杀死肿瘤细胞。
DOI：
10.4049/jimmunol.126.5.1863
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发表时间：
1981
影响因子：
4.4
通讯作者：
S. Russell
中科院分区：
文献类型：
--
作者：
J. Pace;S. Russell
从伴刀豆球蛋白A刺激的脾细胞培养物中获得的富含内毒素的上清液不能激活小鼠腹腔巨噬细胞杀死肿瘤细胞，前提是测定系统不含可检测的内毒素（小于0.125 ng/ml）。相反，增加淋巴因子的量逐渐增加了巨噬细胞对纯化细菌脂多糖（LPS）激活的敏感性。相反，在相对高浓度的LPS（尽管是非活化的）存在下，可以检测到微量淋巴因子的作用。后一种观察结果被用来开发一种高度灵敏的淋巴因子定量测定法。在该试验中，在存在恒定浓度（3 ng/ml）LPS的情况下，在巨噬细胞单层上测试连续稀释的上清液。在这些条件下，淋巴因子剂量-反应曲线呈S形，因此适合使用von Krogh方程的对数转换转换为线性图。活性可以用活性单位来定量和可重复地表示。通过促进纯化，该测定应有助于发展对淋巴因子在巨噬细胞活化以杀死肿瘤细胞中的作用的更精确的生物化学理解。
Lymphokine-rich supernatants from concanavalin A-stimulated spleen cell cultures failed to activate mouse peritoneal macrophages for tumor cell killing, providing that the assay system was free of detectable (less than 0.125 ng/ml) endotoxin. Instead, increasing amounts of lymphokine progressively increased the sensitivity of macrophages to activation by purified bacterial lipopolysaccharide (LPS). Conversely, the effect of minute amounts of lymphokine could be detected in the presence of relatively high (though nonactivating) concentrations of LPS. The latter observation was exploited to develop a highly sensitive, quantitative assay for lymphokine. In this assay, serially diluted supernatants were tested on macrophage monolayers in the presence of a constant concentration (3 ng/ml) of LPS. Under these conditions, the lymphokine dose-response curve was sigmoidal and therefore suitable for conversion to a linear plot using the logarithmic transformation of the von Krogh equation. Activity could be expressed quantitatively and reproducibly in terms of units of activity. By facilitating purification the assay should contribute to the development of a more precise biochemical understanding of the role of lymphokine in macrophage activation for tumor cell killing.