Detoxification of Gramine by the Cereal Aphid Sitobion avenae
Detoxification of Gramine by the Cereal Aphid Sitobion avenae
复制标题
DOI：
10.1007/s10886-009-9603-y
复制
发表时间：
2009-03-01
影响因子：
2.3
通讯作者：
Bi, Jian-Long
中科院分区：
文献类型：
--
作者：
Cai, Qing-Nian;Han, Ying;Bi, Jian-Long
次生代谢物在寄主植物对昆虫的抗性中起着重要作用，而昆虫反过来又可能发展出对抗植物抗性机制的机制。在本研究中，我们研究了谷草胺对玉米蚜虫的毒性以及谷草胺对该生物碱的酶反应。当绿脓杆菌饲喂含有谷草胺的人工饲料时，死亡率呈剂量依赖性。结果表明，对谷氨酰胺的LC50为1.248 mM，对谷氨酰胺的处理显著提高了葡萄球菌的羧酯酶和谷胱甘肽s -转移酶活性。两种酶的活性均与饲粮中谷氨酰胺的浓度呈正相关。此外，麦草碱处理显著提高了麦草碱对过氧化物酶和多酚氧化酶活性的影响；然而，当昆虫暴露于较高水平的膳食谷氨酰胺时，过氧化氢酶活性下降。讨论了谷草胺在寄主植物抗性和葡萄球菌抗性中的潜在作用。
Secondary metabolites play an important role in host plant resistance to insects, and insects, in turn, may develop mechanisms to counter plant resistance mechanisms. In this study, we investigated the toxicity of gramine to the cereal aphid Sitobion avenae and some enzymatic responses of S. avenae to this alkaloid. When S. avenae fed on an artificial diet containing gramine, mortality occurred in a dose-dependent manner. The LC50 of gramine was determined to be 1.248 mM. In response to gramine, S. avenae developed increased activities of carboxylesterase and glutathione S-transferase, two important detoxification enzymes. The activities of both enzymes were positively correlated with the concentration of dietary gramine. In addition, the activities of peroxidase and polypheolic oxidase, two important oxidoreductase enzymes in S. avenae, increased in response to gramine; however, catalase activity decreased when insects were exposed to higher levels of dietary gramine. The potential role of gramine in host plant resistance and S. avenae counter-resistance is discussed.