Differential high-speed digital micromirror device based fluorescence speckle confocal microscopy
Differential high-speed digital micromirror device based fluorescence speckle confocal microscopy
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DOI：
10.1364/ao.49.000497
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发表时间：
2010-01-20
期刊：
影响因子：
1.9
通讯作者：
Walker, John
中科院分区：
文献类型：
--
作者：
Jiang, Shihong;Walker, John
我们报道了一种差分荧光散斑共聚焦显微镜，它利用现代数字微镜器件(DMD)的非常高的帧速率在几分之一秒内获取图像。DMD将一系列预定义的二进制散斑图案投射到样品上，并同时调制返回的荧光的强度。从DMD的“开”和“关”像素反射的荧光分别被相关散斑和防腐散斑调制，在两个CCD相机上平行地形成两幅图像。这两幅图像的和恢复了一幅广域图像，但它们的差异得到了实时的近共焦图像。给出了低数值孔径和高数值孔径下的实验结果。(C)2010年美国光学学会
We report a differential fluorescence speckle confocal microscope that acquires an image in a fraction of a second by exploiting the very high frame rate of modern digital micromirror devices (DMDs). The DMD projects a sequence of predefined binary speckle patterns to the sample and modulates the intensity of the returning fluorescent light simultaneously. The fluorescent light reflecting from the DMD's "on" and "off" pixels is modulated by correlated speckle and anticorrelated speckle, respectively, to form two images on two CCD cameras in parallel. The sum of the two images recovers a widefield image, but their difference gives a near-confocal image in real time. Experimental results for both low and high numerical apertures are shown. (C) 2010 Optical Society of America