GROWTH INHIBITORY PROTEIN(S) IN THE 3T3 CELL PLASMA-MEMBRANE - PARTIAL-PURIFICATION AND DISSOCIATION OF GROWTH INHIBITORY EVENTS FROM INHIBITION OF AMINO-ACID-TRANSPORT
GROWTH INHIBITORY PROTEIN(S) IN THE 3T3 CELL PLASMA-MEMBRANE - PARTIAL-PURIFICATION AND DISSOCIATION OF GROWTH INHIBITORY EVENTS FROM INHIBITION OF AMINO-ACID-TRANSPORT
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DOI：
10.1002/jcp.1041080106
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
GLASER, L
中科院分区：
文献类型：
--
作者：
RABEN, D;LIEBERMAN, MA;GLASER, L
来自[小鼠成纤维细胞] 3 T3细胞的质膜富集部分在生长的G1期早期阻止稀疏3 T3细胞的生长。向稀疏的3 T3细胞添加膜也会导致α-转运速率的降低氨基异丁酸从3 T3细胞的质膜生长抑制蛋白的部分纯化。这些生长抑制蛋白表现为内在膜蛋白，并且不需要膜脂质来进行活性。部分纯化的蛋白质阻止细胞生长而不影响α-半乳糖苷酶的速率。氨基异丁酸转运;转运和细胞生长的抑制不是强制性偶联事件。
A plasma membrane enriched fraction from [mouse fibroblast] 3T3 cells arrests the growth of sparse 3T3 cells early in the G1 phase of growth. Addition of membranes to sparse 3T3 cells also results in a decrease of the rate of transport of .alpha.-aminoisobutyric acid. The growth-inhibitory proteins from plasma membrane of 3T3 cells were partially purified. These growth inhibitory proteins behave as intrinsic membrane proteins and do not require membrane lipids for activity. The partially purified proteins arrest cell growth without affecting the rate of .alpha.-aminoisobutyric transport; inhibition of both transport and cell growth are not obligatorily coupled events.