Distribution of 64Cu in foetal and adult tissues in mice: influence of sodium diethyldithiocarbamate treatment.
Distribution of 64Cu in foetal and adult tissues in mice: influence of sodium diethyldithiocarbamate treatment.
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64Cu 在小鼠胎儿和成年组织中的分布：二乙基二硫代氨基甲酸钠治疗的影响。
DOI：
10.1111/j.1600-0773.1985.tb00041.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
L. Dencker
中科院分区：
文献类型：
--
作者：
S. Jasim;B. Danielsson;H. Tjälve;L. Dencker
将 64Cu（如 64CuCl2）静脉注射给雄性 C57BL 小鼠和不同妊娠阶段的怀孕 C57BL 小鼠。通过放射自显影和伽马能谱研究了 64Cu 在成年动物和胎儿中的分布。还检查了二乙基二硫代氨基甲酸酯 (DEDTC) 处理对动物体内 64Cu 处置的影响。此外，在胚胎肢芽培养系统中研究了铜影响软骨形成的能力。结果显示，在所有时间间隔（5 分钟-24 小时），成年动物的肝脏均强烈吸收 64Cu。在较短的存活时间间隔内，肾皮质、胃肠粘膜、肾上腺、胰腺和红细胞也有摄取。接触Cu可能导致肝脏和肾脏损伤，这可能与这些器官中的强烈蓄积有关。 64Cu 在妊娠的各个阶段都会通过胎盘传递给胎儿，尽管这种情况发生的速度相对较慢。在胎儿体内，肝脏中浓度最高。尽管浓度相对较高，但在鸡肢芽间充质斑点培养系统中观察到铜具有毒性。因此，胎儿畸形和胚胎毒性可能被解释为铜对胚胎结构直接作用的结果，尽管不能排除胎盘和/或母体的影响。用 DEDTC（一种螯合剂）对动物进行预处理或后处理，导致成年动物大多数组织中 64Cu 的浓度显着增加，并且胎儿对该金属的吸收也增加。（摘要截断为 250 字）
64Cu (as 64CuCl2) was given intravenously to male C57BL mice and to pregnant C57BL mice at various stages of gestation. The disposition of the 64Cu in the adult animals and in the foetuses was studied by autoradiography and gamma spectrometry. The effects of treatments with diethyldithiocarbamate (DEDTC) on the disposition of the 64Cu in the animals were also examined. In addition, the ability of Cu to affect chondrogenesis was studied in an embryonic limb bud culture system. The results showed a strong uptake of 64Cu in the liver of the adult animals at all intervals (5 min.-24 hrs). At short survival intervals, there was also an uptake in the kidney cortex, the gastrointestinal mucosa, the adrenal, the pancreas, and the erythrocytes. Exposure to Cu may cause liver and kidney injuries, which may be related to the strong accumulation in these organs. 64Cu passed the placenta to the foetuses at all stages of gestation, although this occurred at a relatively slow rate. Within the foetuses the highest concentrations were found in the liver. Cu was observed to be toxic in the chick limb bud mesenchymal spot culture system although at relatively high concentrations. Foetal malformations and embryotoxicity may therefore be interpreted as a result of direct action of Cu on embryonic structures, although placental and/or maternal influence cannot be excluded. Pre- or posttreatment of the animals with DEDTC, which is a chelating agent, caused a very marked increase in the concentration of 64Cu in most tissues of the adult animals and also an increased foetal uptake of the metal.(ABSTRACT TRUNCATED AT 250 WORDS)