[Evaluation of immunochromatography based rapid detection kit of rotavirus and adenovirus].
[Evaluation of immunochromatography based rapid detection kit of rotavirus and adenovirus].
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基于免疫层析的轮状病毒和腺病毒快速检测试剂盒的评价[J].
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发表时间：
2001
期刊：
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通讯作者：
H. Ushijima
中科院分区：
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作者：
H. Shimizu;L. Li;K. Mitamura;K. Okuyama;Y. Hirai;H. Ushijima
我们研究了Rapidtesta Rota-Adeno （Daiichi Pure Chemicals Co., Ltd, Japan）在临床样品免疫层析同时快速检测A组轮状病毒和腺病毒的有效性。该试剂盒对5株A组轮状病毒、13株腺病毒和其他肠道病毒的反应结果显示，对A组轮状病毒和腺病毒分别形成了红色和蓝色的特异性细胞系。与其他肠道病毒无交叉反应。检出限测定：A组轮状病毒（SA11）为10(4.4)TCID50/ml，血清3型腺病毒为10(4.45)TCID50/ml。试剂盒的检出限与其他免疫层析试剂盒或酶免疫试剂盒相似，比乳胶凝集试验试剂盒的检出限高约10倍。A组轮状病毒和腺病毒与其他免疫层析方法的一致性分别为99.2%（121/122）和98.6%（138/140）。A组轮状病毒与腺病毒的符合率分别为94.5%（69/73）和92.3%（84/91）。试剂盒与其他免疫层析试剂盒的一致性高，病毒检出率高于乳胶凝集试剂盒。Rapidtesta Rota-Adeno能够简单、快速地检测出A组轮状病毒和腺病毒。此外，两种病毒可以通过反应线的颜色差异很容易区分，提示该试剂盒在临床诊断中有用。
We investigated the usefulness of Rapidtesta Rota-Adeno (Daiichi Pure Chemicals Co., Ltd., Japan) for rapid detection of group A rotavirus and adenovirus simultaneously using immunochromatography with clinical samples. In investigation of the reaction of the kit to 5 strains of group A rotavirus, 13 strains of adenovirus and other intestinal viruses, specific lines were formed in red to group A rotavirus and blue to adenovirus. No cross reaction was observed with other intestinal virus. In measurement of detection limit, group A rotavirus (SA11) was detected at 10(4.4)TCID50/ml, serotype 3 adenovirus at 10(4.45)TCID50/ml. The detection limit of the kit was similar to other immunochromatographic assay or enzyme immnoassay kits and approximately 10 times higher than that of kits using latex agglutination test. In comparison with other testing kits in clinical samples, the concordance with other immunochromatographic assay was 99.2% (121/122) in group A rotavirus and 98.6% (138/140) in adenovirus. The rate of concordance with latex agglutination test kit was 94.5% (69/73) in group A rotavirus and 92.3% (84/91) in adenovirus. The kit had high rates of concordance with other immunochromatographic assay kits and higher virus detection rates than those of latex agglutination test kits. Rapidtesta Rota-Adeno is able to detect group A rotavirus and adenovirus simply and rapidly. In addition, the two kinds of virus can be easily differentiated by color difference in reaction lines, suggesting that the kit is useful in clinical diagnosis.