Hybrids of Avena sativa with two diploid wild oats (CIav6956) and (CIav7233) resistant to crown rust
Hybrids of Avena sativa with two diploid wild oats (CIav6956) and (CIav7233) resistant to crown rust
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DOI：
10.1007/s10681-009-0111-5
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发表时间：
2010-07-01
期刊：
影响因子：
1.9
通讯作者：
Dundas, Ian
中科院分区：
文献类型：
--
作者：
Aung, Taing;Zwer, Pamela;Dundas, Ian
经鉴定，CIav 6956和CIav 7233是两份抗冠锈病的二倍体野生燕麦材料。麦长管蚜（Pca.用六倍体燕麦品种Wintaroo和二倍体燕麦Avena strigosa Schreb杂交，获得了两个强有力的间倍性F（1）杂种。登录号CIav 6956。另一个倍性间杂种F（1），命名为“F1-Aa 1”，由Wintaroo和二倍体燕麦登记号CIav 7233的杂交产生。所有三个杂交种都比栽培亲本温塔罗更有活力和更高。3个杂种F（1）均含有双亲的完整染色体（2n = 4x = 28），但自交不结实。杂种的减数分裂分析表明，它们表现出高度的基因组间和基因组内配对。在95-96%的减数分裂细胞中检测到三价构型，并且在所有细胞中存在至少三个二价体。三个杂种F（1）的平均交叉频率为7.2-7.9个/细胞。第四个F（1）杂种随后由二倍体燕麦登记号CIav 7233和Wintaroo之间的杂交产生。从该杂种中产生了一个八倍体（2n = 8x = 56），后代对两个Pca小种都有抗性。八倍体后代的染色体数目在51和54条染色体之间变化。从这些部分八倍体中培育抗冠锈病的染色体附加系是可能的。
Two diploid accessions of wild oat, CIav6956 and CIav7233, were identified as carrying seedling resistance to oat crown rust (caused by Puccinia coronata f. sp. avenae; Pca). Two vigorous interploidy F(1) hybrids were generated from crosses involving the hexaploid oat cultivar Wintaroo and the diploid oat Avena strigosa Schreb. accession CIav6956. An additional interploidy F(1) hybrid, designated "F1-Aa1", was produced from a cross of Wintaroo and the diploid oat accession CIav7233. All three hybrids were more vigorous and taller than the cultivated parent Wintaroo. The three F(1) hybrids contained full chromosome complements from both parents (2n = 4x = 28), but no seeds were obtained when the three F(1) hybrids were selfed. Meiotic analyses of the hybrids indicated that they exhibited a high degree of inter-genome and intra-genome pairing. Trivalent configurations were detected in 95-96% of meiotic cells and a minimum of three bivalents was present in all cells. An average chiasma frequency of 7.2-7.9 per cell was observed for the three F(1) hybrids. A fourth F(1) hybrid was subsequently generated from a cross between the diploid oat accession CIav7233 and Wintaroo. One octaploid (2n = 8x = 56) was generated from this hybrid and progeny were resistant to two Pca races. The chromosome number of the octaploid progeny varied between 51 and 54 chromosomes. Development of a chromosome addition line(s) with the crown rust resistance should be possible from these partial-octaploids.