Free-living and aggregate-associated Planctomycetes in the Black Sea
Free-living and aggregate-associated Planctomycetes in the Black Sea
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DOI：
10.1111/j.1574-6941.2012.01306.x
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发表时间：
2012-05-01
影响因子：
4.2
通讯作者：
Murray, James W.
中科院分区：
文献类型：
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作者：
Fuchsman, Clara A.;Staley, James T.;Murray, James W.
我们研究了未培养的植物种类沿着黑海缺氧带氧化还原梯度的深度剖面分布，以深入了解它们各自的生态位。植物的系统发育与深度和化学特征相关，这意味着系统发育群内的代谢相似。亚氧区样品除Scalindua属的成员明显是自由生活的外，被分成类似于30的>和类似于30的mu - m部分。已知进行厌氧氨氧化过程的Candidatus Scalindua序列形成了两个不同的深度范围不重叠的簇。在高亚硝酸盐/硝酸盐和低铵浓度的上亚氧区，只有97.1%的种群与已命名的物种相似。我们建议将该序列类型命名为Candidatus Scalindua richardsii。第二簇的序列与Candidatus Scalindua sorokinii相似，在低亚氧带的高铵低亚硝酸盐条件下存在。从sulfidic带（1000相似于m深度）获得的序列来自两个未表征的plananctomycetacia簇和三个潜在的新属，以及来自未培养的OP3门的序列。
We examined the distribution of uncultured Planctomycetes phylotypes along depth profiles spanning the redox gradient of the Black Sea suboxic zone to gain insight into their respective ecological niches. Planctomycetes phylogeny correlated with depth and chemical profiles, implying similar metabolisms within phylogenetic groups. A suboxic zone sample was split into >similar to 30 and similar to 30 similar to mu m fraction except for members of the Scalindua genus, which were apparently free-living. Sequences from Candidatus Scalindua, known to carry out the anammox process, formed two distinct clusters with nonoverlapping depth ranges. One cluster, only 97.1% similar to the named species, was present at high nitrite/nitrate and low ammonium concentrations in the upper suboxic zone. We propose this sequence type be named Candidatus Scalindua richardsii. A second cluster, containing sequences more similar to Candidatus Scalindua sorokinii, was present at high ammonium and low nitrite conditions in the lower suboxic zone. Sequences obtained from the sulfidic zone (1000 similar to m depth) yielded Planctomycetes from two uncharacterized Planctomycetacia clusters and three potentially new genera as well as sequences from the uncultured OP3 phylum.