Potential loss of clubroot resistance genes from donor parent Brassica rapa subsp rapifera (ECD 04) during doubled haploid production
Potential loss of clubroot resistance genes from donor parent Brassica rapa subsp rapifera (ECD 04) during doubled haploid production
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DOI：
10.1111/ppa.12816
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发表时间：
2018-05-01
期刊：
影响因子：
2.7
通讯作者：
Feindel, D.
中科院分区：
文献类型：
--
作者：
Fredua-Agyeman, R.;Hwang, S. -F.;Feindel, D.
来自油菜/甘蓝型油菜的根瘤病抗性已在加拿大阿尔伯塔省的一些地区被油菜原生生物油菜根瘤病新品系的出现所克服。对来自油菜亚种的112个加倍单倍体品系进行了抗病性鉴定。Rapifera(欧洲根瘤分化(ECD)04)。这些品系与代表老的致病类型2、3、5、6和8的5个单孢菌株和代表新品系的15个田间群体进行了比较。病情严重度指数(ID%)结果表明，没有一株系对新致病类型5X(田间群体L-G1、L-G2、L-G3)和D-G3表现抗性或中抗，其余11个新品系的抗性和中抗率为3~42%。使用由旧致病类型3(约.13.5%)，接种新病原菌后，根瘤病严重程度提高了300-600%。本研究的一个重要发现是，ECD04对所有新老油菜品系都表现出绝对抗性，而甘蓝型油菜虽然对所有旧致病类型都具有抗性，但只对约50%的新品系表现出抗性。同样，在本研究中评估的所有抗性商业油菜品种都对87%的白菜型油菜新菌株敏感。分子生物学数据表明，孟德尔和油菜品种对根肿病抗性的丧失部分是由于ECD 04不遗传A08染色体上的Crr1基因所致。
Clubroot resistance derived from the oilseed rape/canola Brassica napus Mendel' has been overcome in some fields in Alberta, Canada, by the emergence of new' strains of the protist Plasmodiophora brassicae. Resistance to the pathogen was assessed in 112 doubled haploid (DH) lines, derived from B.rapa subsp. rapifera (European clubroot differential (ECD) 04). The lines were evaluated against five single-spore isolates representing the old' pathotypes 2, 3, 5, 6 and 8, and 15 field populations representing new strains of P.brassicae. The disease severity index (ID%) data revealed that none of the DH lines were resistant or moderately resistant to the new pathotype 5X (field populations L-G1, L-G2, L-G3) and D-G3, while 3-42% were resistant or moderately resistant to the other 11 new strains. Using the mean ID induced by the old pathotype 3 (approx. 13.5%) as the baseline, clubroot severity increased by 300-600% when inoculated with the new pathotypes. A significant finding of this study was the fact that ECD 04 showed absolute resistance to all of the old and new P.brassicae strains while the B.napus Mendel', although resistant to all of the old pathotypes, was resistant to only about 50% of the new strains. Similarly, all of the selected clubroot-resistant commercial canola cultivars evaluated in this study were susceptible to 87% of the new P.brassicae strains. The molecular data revealed that the breakdown of clubroot resistance in Mendel and the canola cultivars was in part due to the non-inheritance of the Crr1 gene on the A08 chromosome from ECD 04.