Hsp90 as a capacitor of phenotypic variation
Hsp90 as a capacitor of phenotypic variation
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DOI：
10.1038/nature749
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发表时间：
2002-06-06
期刊：
影响因子：
64.8
通讯作者：
Lindquist, S
中科院分区：
文献类型：
--
作者：
Queitsch, C;Sangster, TA;Lindquist, S
热休克蛋白90 （Hsp90）伴随着许多调节蛋白的成熟，并在果蝇黑腹果蝇中缓冲形态发生途径的遗传变异。在强制近亲繁殖的物种（如果蝇）和自交受精的物种（如拟南芥）之间，遗传变异的水平和模式差别很大。此外，植物的发育更具可塑性，与环境因素有关。本文报道，在拟南芥和重组自交系中，降低Hsp90功能会产生一系列依赖于潜在遗传变异的形态表型。Hsp90对遗传变异的缓冲和释放作用的强度和广度表明它可能对进化过程有影响。我们还表明，Hsp90影响对环境线索的形态发生反应，并缓冲随机过程不稳定效应的正常发育。操纵Hsp90的缓冲能力为利用隐性遗传变异和阐明基因型、环境和决定表型的随机事件之间的相互作用提供了工具。
Heat-shock protein 90 (Hsp90) chaperones the maturation of many regulatory proteins and, in the fruitfly Drosophila melanogaster, buffers genetic variation in morphogenetic pathways. Levels and patterns of genetic variation differ greatly between obligatorily outbreeding species such as fruitflies and self-fertilizing species such as the plant Arabidopsis thaliana. Also, plant development is more plastic, being coupled to environmental cues. Here we report that, in Arabidopsis accessions and recombinant inbred lines, reducing Hsp90 function produces an array of morphological phenotypes, which are dependent on underlying genetic variation. The strength and breadth of Hsp90's effects on the buffering and release of genetic variation suggests it may have an impact on evolutionary processes. We also show that Hsp90 influences morphogenetic responses to environmental cues and buffers normal development from destabilizing effects of stochastic processes. Manipulating Hsp90's buffering capacity offers a tool for harnessing cryptic genetic variation and for elucidating the interplay between genotypes, environments and stochastic events in the determination of phenotype.