MONONUCLEAR CELL SUBPOPULATIONS IN HUMAN FOLLICULAR-FLUID FROM STIMULATED CYCLES
MONONUCLEAR CELL SUBPOPULATIONS IN HUMAN FOLLICULAR-FLUID FROM STIMULATED CYCLES
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DOI：
10.1111/j.1600-0897.1990.tb00655.x
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发表时间：
1990-03-01
影响因子：
3.6
通讯作者：
HERRUZO, AJ
中科院分区：
文献类型：
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作者：
CASTILLA, JA;SAMPALO, A;HERRUZO, AJ
对人卵泡液淋巴细胞亚群的研究为评价卵巢的免疫学特性提供了可能。我们调查了10例体外受精（IVF）患者在腹腔镜检查时获得的FF和外周血中的单核细胞亚群。以周期中期非妊娠外周血作为对照。在FF中观察到单核细胞（CD 14+）比例显著增加。尽管FF富含CD 8+淋巴细胞，但观察到CD 4+淋巴细胞比例降低。通过CD 4 + CD 45 R-表型鉴定的FF中的“记忆”T细胞以2：1的比例超过“初始”T细胞（CD 4 + CD 45 R+），这与对照血液样品产生的比例（1：1）不同。FF中活化T细胞（CD 3 + HLA-DR+细胞）的百分比显著增加。IVF患者外周血淋巴细胞亚群的变化与FF相似，但不显著，支持淋巴细胞在卵巢生理中起重要作用的观点。
The study of lymphocyte subsets from human follicular fluid (FF) provides an opportunity to evaluate immunological features of the ovary. We investigated the mononuclear cell subsets in FF and peripheral blood obtained at the time of laparoscopy from ten in vitro fertilization (IVF) patients. Midcycle nonpregnant peripheral blood was used as the control. A marked increase in the proportion of monocytes (CD14+) was observed in FF. Although FF was enriched with CD8+ lymphocytes, a decrease in the proportion of CD4+ lymphocytes was observed. "Memory" T cells in FF, identified by the CD4+ CD45R- phenotype, predominated over "naive" T cells (CD4+ CD45R+) at a ratio of 2:1, which differs from the ratio yielded by control blood samples (1:1). The percentage of activated T cells (CD3+ HLA-DR+ cell) increased significantly in FF. When lymphocyte subsets were studied in the peripheral blood of IVF patients, changes similar to but less significant than those in FF were found. These data support the concept that lymphocytes play an important role in ovarian physiology.