Effects of polyhydroxy compounds on beetle antifreeze protein activity.
Effects of polyhydroxy compounds on beetle antifreeze protein activity.
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DOI：
10.1016/j.bbapap.2008.10.011
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Wen X
中科院分区：
文献类型：
--
作者：
Amornwittawat N;Wang S;Banatlao J;Chung M;Velasco E;Duman JG;Wen X
抗冻蛋白（AFPs）非综合地降低溶液的非平衡冰点，并产生被称为热滞后（TH）的熔点和冰点之间的差异。一些低分子质量的溶质会影响TH值。采用差示扫描量热法（DSC）系统研究了多羟基化合物（包括多元醇和碳水化合物）对加拿大石斛甲胎蛋白的TH增强作用。羟基的数量决定了具有1到5个羟基的多羟基化合物的摩尔增强效果。但是，上述规则不适用于含有5个以上羟基的多羟基化合物。最有效的多羟基增强剂是海藻糖。在增强子的组合中，最强的增强子在决定TH增强方面起主要作用。讨论了高效AFP增强子鉴定的机制见解。
Antifreeze proteins (AFPs) noncolligatively depress the nonequilibrium freezing point of a solution and produce a difference between the melting and freezing points termed thermal hysteresis (TH). Some low-molecular-mass solutes can affect the TH values. The TH enhancement effects of selected polyhydroxy compounds including polyols and carbohydrates on an AFP from the beetle Dendroides canadensis were systematically investigated using differential scanning calorimetry (DSC). The number of hydroxyl groups dominates the molar enhancement effectiveness of polyhydroxy compounds having one to five hydroxyl groups. However, the above rule does not apply for polyhydroxy compounds having more than five hydroxyl groups. The most efficient polyhydroxy enhancer identified is trehalose. In a combination of enhancers the strongest enhancer plays the major role in determining the TH enhancement. Mechanistic insights into identification of highly efficient AFP enhancers are discussed.