STRUCTURAL-CHANGES OCCURRING DURING ATRESIA IN SHEEP OVARIAN FOLLICLES
STRUCTURAL-CHANGES OCCURRING DURING ATRESIA IN SHEEP OVARIAN FOLLICLES
复制标题
DOI：
10.1007/bf00218150
复制
发表时间：
1976-01-01
影响因子：
3.6
通讯作者：
MOOR, RM
中科院分区：
文献类型：
--
作者：
HAY, MF;CRAN, DG;MOOR, RM
应用组织学、组织化学和超微结构技术研究了绵羊Graafian卵泡原发性、继发性和三级闭锁的结构变化，原发性闭锁卵泡中，沿窦缘沿着明显的空泡，代表肿胀的内质网，颗粒细胞排列紊乱，核固缩。在颗粒膜内观察到吞噬细胞，其数量随着闭锁的进展而增加，被认为是转化的颗粒细胞。即使在非闭锁的卵泡中，也有少量窦空泡和偶见固缩的细胞核。在继发性闭锁时，核固缩的细胞数量大量增加;许多核挤压并融合形成特征性的Feulgen阳性闭锁小体，发现这些小体沿着胃窦腔的边缘。这些体通常具有高达15 μ m的直径，但偶尔达到高达400 μ m。第二个变性区域经常出现在颗粒膜上，即基底膜的2或3层细胞层，三级闭锁中颗粒细胞的脱落就发生在这一水平。与颗粒膜相反，在继发性闭锁期间，卵丘仅有轻微的退化迹象。在三级闭锁中，颗粒膜高度紊乱;闭锁体的数量通常比早期少。基底层基本上保持完整。正是在这个阶段，第一个明显的退化迹象发生在内膜。尽管卵丘有些解体，但卵母细胞的完整性得以保持，其核仍呈囊状。卵泡膜微循环的变化可能在闭锁中起关键作用：邻近非闭锁卵泡的基底层，存在发育良好的毛细血管网络，其随着闭锁的进展而显著减少。
The structural changes that characterize primary, secondary and tertiary atresia in sheep Graafian follicles were studied by histological, histochemical and ultrastructural techniques.In primary atresia vacuoles representing swollen endoplasmic reticulum are prominent along the antral border together with disorganized granulosa cells containing pyknotic nuclei. Phagocytic cells, which increase in number as atresia progresses, were seen within the membrana granulosa and are considered to be transformed granulosa cells. Even in follicels classified as non-atretic, a few antral vacuoles and occasional pyknotic nuclei are present. During secondary atresia there is a large increase in the number of cells with pyknotic nuclei; many of these nuclei extruded and had fused to form the characteristic Feulgen-positive atretic bodies found along the edge of the antral cavity. These bodies usually have a diameter of up to 15 .mu.m but occasionally reached as much as 400 .mu.m. A 2nd area of degeneration is frequently present in the membrana granulosa, 2 or 3 cell layers from the basal lamina, and it is at this level that exfoliation of granulosa cells occurs in tertiary atresia. In contrast to the membrana granulosa, there are during secondary atresia, only slight indications of degeneration in the cumulus. In tertiary atresia the membrana granulosa is highly disorganized; the atretic bodies are often fewer in number than at earlier stages. The basal lamina remains essentially intact. It is at this stage that the 1st clear signs of degeneration occur in the theca interna. Despite some disintegration of the cumulus, the integrity of the oocyte is maintained and its nucleus remains vesicular. Changes in the thecal microcirculation may play a key role in atresia: adjacent to the basal lamina of non-atretic follicles, there is a well-developed capillary network which is significantly reduced as atresia progresses.