The structure and composition of aleurone grains in the barley aleurone layer
The structure and composition of aleurone grains in the barley aleurone layer
复制标题
大麦糊粉层中糊粉粒的结构和组成
DOI：
10.1007/bf00386828
复制
发表时间：
2004
期刊：
影响因子：
4.3
通讯作者：
N. A. Pyliotis
中科院分区：
文献类型：
--
作者：
J. Jacobsen;R. Knox;N. A. Pyliotis
细胞化学方法与光学和电子显微镜相结合，用来确定大麦糊粉层糊粉粒中包裹体的性质。包涵体有两种类型：(1)未被膜包裹的球体腔内的球体：由于菲丁的存在而染成红色的甲苯胺蓝，并有脂肪染色；(2)蛋白质-碳水化合物小体，染成绿色的甲苯胺蓝。使用戊二醛和高锰酸盐固定的组织，详细描述了在电子显微镜下看到的球体和蛋白质-碳水化合物小体的特征。蛋白质-碳水化合物小体经六色银标记法鉴定，这不是由碳水化合物引起的，而是由甲苯胺蓝染成绿色的一些成分引起的，这种物质也出现在细胞壁中靠近细胞质的一条细带中。讨论了这两个物体的特征与它们在以前的电子显微镜研究中身份的明显混淆有关。
Cytochemical methods have been used in conjunction with light and electron microscopy to determine the nature of the inclusions in aleurone grains of barley aleurone layers. Two kinds of inclusions were found: (1) Globoids within globoid cavities which were not enclosed by a membrane: the globoids stained red with toluidin blue due to the presence of phytin, and with lipid stains; (2) Protein-carbohydrate bodies which stained green with toluidin blue. The characteristics of globoids and protein-carbohydrate bodies as seen in the electron microscope are described in detail using both glutaraldehyde- and permanganatefixed tissues. The protein-carbohydrate body was identified by silver-hexaminestaining; this was not caused by carbohydrate but by some component which stained green in toluidin blue and which also occurred in cell walls in a thin band adjacent to the cytoplasm. The characteristics of both bodies are discussed in relation to apparent confusion in their identities in previous electron-microscope studies.