Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography
Three dimensional visualization of engineered bone and soft tissue by combined x-ray micro-diffraction and phase contrast tomography
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DOI：
10.1088/0031-9155/59/1/189
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发表时间：
2014-01-06
影响因子：
3.5
通讯作者：
Mastrogiacomo, Maddalena
中科院分区：
文献类型：
--
作者：
Cedola, Alessia;Campi, Gaetano;Mastrogiacomo, Maddalena
计算机X射线位相对比显微断层成像是对组织工程骨形态进行三维和无损分析的最有价值的工具。我们使用安装在Elettra同步加速器(意大利的里雅斯特)SYRMEP光束线上的Talbot干涉仪对骨和软结缔组织进行了精确的3D重建，并在体内多孔性支架内再生。首次将X射线层析重建与X射线扫描显微衍射测量相结合，对参与生物矿化过程的不同组织进行了详尽的识别。结果，我们能够详细地研究组织中的不同密度，区分无定形磷酸钙和胶原基质的3D结构。我们的实验方法使我们能够更深入地了解胶原基质在有机-矿物转变中的作用，这是开发新的生物启发复合材料的关键问题。
Computed x-ray phase contrast micro-tomography is the most valuable tool for a three dimensional (3D) and non destructive analysis of the tissue engineered bone morphology. We used a Talbot interferometer installed at SYRMEP beamline of the ELETTRA synchrotron (Trieste, Italy) for a precise 3D reconstruction of both bone and soft connective tissue, regenerated in vivo within a porous scaffold. For the first time the x-ray tomographic reconstructions have been combined with x-ray scanning micro-diffraction measurement on the same sample, in order to give an exhaustive identification of the different tissues participating to the biomineralization process. As a result, we were able to investigate in detail the different densities in the tissues, distinguishing the 3D organization of the amorphous calcium phosphate from the collagen matrix. Our experimental approach allows for a deeper understanding of the role of collagen matrix in the organic-mineral transition, which is a crucial issue for the development of new bio-inspired composites.