RECORDING SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DISSOCIATION AND PHOSPHORYLATION CONSTANTS FOR INHIBITION OF ACETYLCHOLINESTERASE BY ORGANOPHOSPHATES IN PRESENCE OF SUBSTRATE
RECORDING SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DISSOCIATION AND PHOSPHORYLATION CONSTANTS FOR INHIBITION OF ACETYLCHOLINESTERASE BY ORGANOPHOSPHATES IN PRESENCE OF SUBSTRATE
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DOI：
10.1021/bi00739a026
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发表时间：
1973-01-01
期刊：
影响因子：
2.9
通讯作者：
OBRIEN, RD
中科院分区：
文献类型：
--
作者：
HART, GJ;OBRIEN, RD
杰弗里J.哈特和理查德D.奥布莱恩 * 摘要：本文介绍了一种用记录式分光光度计测定有机磷酸酯在体外抑制酯酶的亲和常数、磷酸化常数和双分子反应常数的实验方法。讨论了相关方程的理论背景和推导过程。用该方法研究了温度对缓蚀作用的影响
Geoffrey J. Hart and Richard D. O’Brien* abstract: An experimental procedure, based on the use of a recording spectrophotometer, has been developed for deter-mination of affinity, phosphorylation, and bimolecular reac-tion constants for the inhibition of an esterase by organophosphates in vitro. The theoretical background and derivation of the relevant equations are discussed. Themethod has been applied to a study of the effect of temperature on the inhibition