MOLECULAR ANALYSIS OF THE ARMADILLO-LOCUS - UNIFORMLY DISTRIBUTED TRANSCRIPTS AND A PROTEIN WITH NOVEL INTERNAL REPEATS ARE ASSOCIATED WITH A DROSOPHILA SEGMENT POLARITY GENE
MOLECULAR ANALYSIS OF THE ARMADILLO-LOCUS - UNIFORMLY DISTRIBUTED TRANSCRIPTS AND A PROTEIN WITH NOVEL INTERNAL REPEATS ARE ASSOCIATED WITH A DROSOPHILA SEGMENT POLARITY GENE
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DOI：
10.1101/gad.3.1.96
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发表时间：
1989-01-01
影响因子：
10.5
通讯作者：
SCHEDL, P
中科院分区：
文献类型：
--
作者：
RIGGLEMAN, B;WIESCHAUS, E;SCHEDL, P
在果蝇胚胎发育过程中，体节极性基因是每个体节内形成特定模式域所必需的。Armadillo（arm）基因的突变主要影响节段的后部，并导致在该区域内产生前部结构。为了研究这些效应的分子基础，我们克隆了臂区，并通过种系转化鉴定了该基因。臂基因产生两种类型的非常丰富的3.2 kb的成绩单，不同的只是在他们的第一个外显子。这些RNA似乎是由独立的转录起始形成的，但在整个发育过程中具有相似的表达模式。这两个手臂转录本中包含的胚胎，三龄幼虫，和成年卵巢，表明手臂可能需要在所有细胞中的几乎所有的细胞类型。此外，手臂转录本均匀分布在胚胎片段中，因此与其胚胎表型相关的区域模式缺陷可能是手臂与其他局部因素之间相互作用的结果。两个臂RNA编码相同的91-kD多肽。该蛋白没有可能的分泌或跨膜区域，并含有一系列在序列、长度和间隔上保守的新型内部重复序列。考虑到这些结果和以前的遗传观察，我们讨论了手臂基因在图案形成过程中的潜在作用。
During Drosophila embryogenesis, the segment polarity genes are required for the formation of specific pattern domains within each segment. Mutations in the armadillo (arm) gene primarily affect the posterior part of the segment and lead to the production of anterior structures within this region. To examine the molecular basis for these effects, we have cloned the arm region and identified the gene by germ-line transformation. The arm gene produces two types of very abundant 3.2-kb transcripts that differ only in their first exons. These RNAs appear to be formed by independent transcriptional initiation but have similar patterns of expression throughout development. Both arm transcripts are present in virtually all of the cell types contained in embryos, third-instar larvae, and adult ovaries, suggesting that arm may be required in all cells. In addition, the arm transcripts are uniformly distributed in embryonic segments, so the regional pattern defects associated with its embryonic phenotype may result from interactions between arm and other localized factors. Both arm RNAs encode the same 91-kD polypeptide. This protein has no probable secretory or membrane-spanning regions and contains a series of novel internal repeats that are conserved in sequence, length, and spacing. Considering these results and previous genetic observations, we discuss potential role for the arm gene in pattern formation processes.