Electrochemical Quartz Crystal Microbalance Study of Direct Bioelectrocatalytic Reduction of Bilirubin Oxidase
Electrochemical Quartz Crystal Microbalance Study of Direct Bioelectrocatalytic Reduction of Bilirubin Oxidase
复制标题
胆红素氧化酶直接生物电催化还原的电化学石英晶体微天平研究
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
K. Kano
中科院分区：
文献类型：
--
作者：
Yuji Kamitaka;S. Tsujimura;T. Ikeda;K. Kano
使用石英晶体微天平 (QCM) 测量和无介体的 BOD 催化 O2 还原电流的电化学测量，研究了胆红素氧化酶 (BOD) 在 Au 电极上的吸附行为。 QCM 测量支持在 pH 5 至 9 的金电极上单层吸附 BOD。然而，催化电流密度远小于亲水边缘平面型碳电极或活性 BOD 的计算值。电化学 QCM (EQCM) 测量结果表明金电极上存在一定的变性。
Adsorption behavior of bilirubin oxidase (BOD) on Au electrodes was studied using quartz crystal microbalance (QCM) measurements and electrochemical measurements of BOD-catalyzed reduction current of O2 without mediators. The QCM measurements supported the monolayer adsorption of BOD on Au electrodes at pH 5 to 9. The catalytic current density was, however, much smaller than that at hydrophilic edge plane-type carbon electrodes or than the calculated value for active BOD. Some denaturation is suggested on Au electrodes, which was supported by electrochemical QCM (EQCM) measurements.