Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements
Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements
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DOI：
10.1021/jf0502698
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发表时间：
2005-05-18
影响因子：
6.1
通讯作者：
Schaich, K
中科院分区：
文献类型：
--
作者：
Prior, RL;Wu, XL;Schaich, K
综述了可用于测量抗氧化能力的方法，介绍了测定方法的一般化学原理、检测到的分子类型以及每种方法最重要的优点和缺点。本概述为食品、营养品和膳食补充剂行业开发标准化抗氧化能力方法提供了基础和原理。根据对 2004 年第一届国际抗氧化剂方法大会上提出的数据和文献的评估，以及对抗氧化剂潜在最终用途的考虑，建议考虑对三种测定的程序和应用进行标准化：氧自由基吸收能力 (ORAC) 测定、Folin-Ciocalteu 方法，以及可能的 Trolox 等效抗氧化能力 (TEAC) 测定。 ORAC代表了与人类生物学最相关的氢原子转移（HAT）反应机制。 Folin-Ciocalteu 方法是一种基于电子转移 (ET) 的测定方法，可给出还原能力，通常以酚含量表示。 TEAC 测定代表了第二种基于 ET 的方法。随着人们对其他一些激进来源及其对人类生物学的重要性的了解越来越多，将来可能需要考虑其他分析方法。
Methods available for the measurement of antioxidant capacity are reviewed, presenting the general chemistry underlying the assays, the types of molecules detected, and the most important advantages and shortcomings of each method. This overview provides a basis and rationale for developing standardized antioxidant capacity methods for the food, nutraceutical, and dietary supplement industries. From evaluation of data presented at the First International Congress on Antioxidant Methods in 2004 and in the literature, as well as consideration of potential end uses of antioxidants, it is proposed that procedures and applications for three assays be considered for standardization: the oxygen radical absorbance capacity (ORAC) assay, the Folin-Ciocalteu method, and possibly the Trolox equivalent antioxidant capacity (TEAC) assay. ORAC represent a hydrogen atom transfer (HAT) reaction mechanism, which is most relevant to human biology. The Folin-Ciocalteu method is an electron transfer (ET) based assay and gives reducing capacity, which has normally been expressed as phenolic contents. The TEAC assay represents a second ET-based method. Other assays may need to be considered in the future as more is learned about some of the other radical sources and their importance to human biology.