Ability of excised root and stem pieces of maize, cowpea and soybean to cause germination of Striga hermonthica seeds
Ability of excised root and stem pieces of maize, cowpea and soybean to cause germination of Striga hermonthica seeds
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DOI：
10.1016/s0261-2194(02)00177-1
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发表时间：
2003-03-01
期刊：
影响因子：
2.8
通讯作者：
Ahonsi, MO
中科院分区：
文献类型：
--
作者：
Emechebe, AM;Ahonsi, MO
草鱼实验结果差异很大是很常见的。用切根法对寄主和非寄主植物品种的萌发进行体外筛选尤其不敏感。本文研究了断根技术对同一作物品种诱导的黑麦草种子群体发芽率显著差异的影响。玉米(杂交种Var 8338-1)、豌豆(Var IT81D-994)和大豆(Var TGX 1448-2E)的根和茎切段均能促进黑麦草种子萌发。玉米和豌豆茎段的发芽率显著高于根段。用5毫升蒸馏水浸湿处理过的稻瘟病菌种子的滤纸上，玉米和大豆的刺激物均能显著提高该寄生虫种子的发芽率，而不考虑所测试的植物部分。无论植物种类和植物部位如何，切花后立即开始提取促萌剂，种子发芽率显著高于3h后开始提取。用无菌水处理种子，然后用无菌水提取发芽促进剂，通常比用无菌蒸馏水处理的种子发芽率高。对这些结果进行了讨论，并对如何减少切根法测定寄主和诱捕作物萌发刺激物的结果的变异性提出了建议。(C)2003爱思唯尔科学有限公司。保留所有权利。
High variation in results from Striga hermonthica experiments is common. The cut-root assay for in vitro screening of host and non-host plant cultivars for germination of S. hermonthica is particularly insensitive. In this study, some factors of the cut-root technique that could effect significant variation in germination percentage of a population of S. hermonthica seeds induced by the same crop cultivar were studied. It was found that excised pieces of both root and stem of maize (hybrid var 8338-1), cowpea (var IT81D-994) and soybean (var TGx 1448-2E) stimulated the germination of S. hermonthica seeds. Germination percentages obtained with maize and cowpea stem pieces were significantly higher than those produced by root pieces. Moistening filter paper on which conditioned Striga seeds were subjected to germination stimulants with 5 ml of distilled water resulted in significantly higher germination of the parasite's seeds induced by stimulants from both maize and soybean, than adding 3 ml of water, regardless of the plant part tested. Starting germination stimulant extraction immediately after cutting plant parts gave significantly higher germination percentage of S. hermonthica seeds than starting 3 h later, regardless of crop species and plant part tested. Conditioning S. hermonthica seeds and subsequent extraction of germination stimulant with non-sterile water generally resulted in higher germination percentage of S. hermonthica seeds than with sterile distilled water. These results are discussed and suggestions made about how to reduce variability of results of the cut-root method of in vitro assaying of germination stimulant production by hosts and trap crops of S. hermonthica. (C) 2003 Elsevier Science Ltd. All rights reserved.