Development of a quantitative analysis method for measuring the change in vascular structure of malignant tumors in small experimental animals
Development of a quantitative analysis method for measuring the change in vascular structure of malignant tumors in small experimental animals
复制标题
小型实验动物恶性肿瘤血管结构变化定量分析方法的建立
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
I. Kashima
中科院分区：
文献类型：
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作者：
T. Sakurai;R. Kawamata;I. Kashima
目的建立一种详细的、定量的X线影像学评价方法，用于分析小动物恶性肿瘤的血管结构。方法采用微聚焦管X线成像系统和计算机X线摄影系统，在7倍放大率下直接采集图像。应用数学形态学滤波器提取血管结构模式，然后用图像分析软件对数字图像中血管结构的各种参数进行定量分析。评价包括以下三个阶段：用图形软件绘制的数字图像的评价，用铜丝制作的血管结构模型体模的数字X线图像的评价，以及小鼠肿瘤模型的血管造影的评价。与此相反，非血管结构参数的厚度，长度和面积的测试图表的增加而减少。模型体模的数字X射线图像的分析结果与数字测试图图像的分析结果相似。肿瘤模型小鼠的血管结构参数值高于对照小鼠。与此相反，测量的非血管参数的参数值低于在肿瘤模型小鼠比在对照mouse.ConclusionsThe结果，我们的初步调查表明，我们的定量X射线成像评价方法可能是有用的分析血管结构的肿瘤在小的实验动物。
ObjectivesThe aim of this study was to develop a detailed and quantitative X-ray imaging evaluation method for analyzing the vascular structure of malignant tumors in small experimental animals.MethodsA microfocus tube X-ray imaging system and a computed radiography system were used to capture images directly at 7× magnification. Various parameters of the vascular structures in the digital images were quantitatively analyzed with image analysis software after the vascular structure patterns were extracted by a mathematical morphology filter. The evaluation included the following three stages: assessment of the digital images drawn with graphics software, evaluation of digital X-ray images of vascular structure model phantoms made from copper wire, and assessment of the angiography of the mouse tumor model.ResultsIn the quantitative analysis of the digital images, each vascular structure parameter increased as the thickness, length, and area of the test chart increased. In contrast, nonvascular structure parameters decreased with increases in the thickness, length, and area of the test chart. The results of the analysis of the digital X-ray images of model phantoms were similar to those of the digital test chart image. Vascular structure parameter values were higher in the tumor model mouse than in the control mouse. In contrast, values of parameters that measured the nonblood vessel parameters were lower in the tumor model mouse than in the control mouse.ConclusionsThe results of our preliminary investigation suggest that our quantitative X-ray imaging evaluation method may be useful for analyzing the vascular structure of tumors in small experimental animals.
影响因子：
11.2
作者：
Tang, Y;Kim, M;Weissleder, R
通讯作者：
Weissleder, R