Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo
Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo
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DOI：
10.1111/j.0022-202x.2004.23550.x
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发表时间：
2005-01-01
影响因子：
6.5
通讯作者：
Chung, JH
中科院分区：
文献类型：
--
作者：
Chen, Z;Seo, JY;Chung, JH
光老化皮肤的网状真皮上层含有弹性物质。这种现象通常被称为日光性弹性组织变性。在这项研究中，我们研究了热对弹性纤维的两个主要成分原弹性蛋白和弹性蛋白-1的表达，以及对基质金属蛋白酶（MMP）-12，最活跃的MMP对弹性蛋白，在人体皮肤中的影响。发现热增加表皮和真皮中的原弹性蛋白mRNA和蛋白质表达。原纤维蛋白-1 mRNA和蛋白表达增加，热在表皮，但在真皮减少。我们发现，在热处理前用N-乙酰半胱氨酸或染料木素预处理皮肤24小时抑制了热诱导的弹性蛋白原表达，但不抑制弹性蛋白-1。这些数据表明，活性氧簇可能发挥作用，在原弹性蛋白表达的热量，但不是在repeatin-1的表达。我们还发现，热处理增加MMP-12 mRNA和蛋白质在人体皮肤中的表达。我们的研究结果表明，异常生产的原弹性蛋白和弹性蛋白在人体皮肤中的热量和降解的各种MMP，如MMP-12，可能有助于在光老化的皮肤中的弹性组织变性材料的积累。
Photoaged skin contains elastotic materials in the upper reticular dermis. This phenomenon is commonly known as solar elastosis. In this study, we investigated the effects of heat on the expression of tropoelastin and fibrillin-1, two main components of elastic fibers, and on matrix metalloproteinase (MMP)-12, the most active MMP against elastin, in human skin in vivo. Heat was found to increase tropoelastin mRNA and protein expression in the epidermis and in the dermis. Fibrillin-1 mRNA and protein expression were increased by heat in the epidermis, but were decreased in the dermis. We found that pre-treatment of skin with N-acetyl cysteine or genistein for 24 h prior to heat treatment inhibited the heat-induced expression of tropoelastin, but not of fibrillin-1. These data indicate that reactive oxygen species may play a role in tropoelastin expression by heat, but not in fibrillin-1 expression. We also found that heat treatment increases MMP-12 mRNA and protein expression in human skin. Our results suggest that the abnormal production of tropoelastin and fibrillin by heat in human skin and that their degradation by various MMP, such as MMP-12, may contribute to the accumulation of elastotic material in photoaged skin.