Placental transfer of benzo(a)pyrene and its hydrophilic metabolites in the guinea pig.
Placental transfer of benzo(a)pyrene and its hydrophilic metabolites in the guinea pig.
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DOI：
10.1111/j.1600-0773.1986.tb00108.x
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
I. Kihlström
中科院分区：
文献类型：
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作者：
I. Kihlström
用豚鼠胎盘灌流技术研究了苯并（a）芘（BP）及其亲水性代谢产物（mBP）在母体中的胎盘转运。停止输注时，通过脐静脉从子宫内的完整胎仔中采集血样。当使用来自成年豚鼠的肝素化血液作为灌注介质时，BP的胎盘转移速率比mBP的胎盘转移速率快。当使用来自胎儿的肝素化血液代替来自成人的血液时，转移率基本相同。用右旋糖酐溶液作为灌注介质，没有看到BP或mBP的转移。使用不同浓度的白蛋白溶液，只有可忽略不计的BP或mBP渗透胎盘。灌注血浆中BP的浓度显著高于胎儿血浆中的浓度，与灌注血浆中MBP的浓度显著低于胎儿血浆相反。它的结论是，在妊娠后期胎儿暴露的MBP是由于胎儿代谢转换的BP比胎儿暴露的MBP产生的母体酶活性。
The placental transfer of benzo(a)pyrene, (BP) and its hydrophilic metabolites, (mBP) have been studied in the guinea pig by a placental perfusion technique during constant infusion of BP to the dam. In connection with stop of infusion, blood samples were withdrawn from intact foetuses remaining in utero via the umbilical vein. The placental transfer rate of BP is faster compared with that of mBP when heparinized blood from adult guinea pigs is used as perfusion media. Transfer rate was essentially the same when heparinized blood from foetuses was used instead of blood from adults. With a dextran solution as perfusion media no transfer of BP or mBP could be seen. With albumin solutions of different concentrations only negligible amounts of BP or mBP penetrated the placenta. The concentration of BP in perfusion plasma was significantly higher than that in the foetal plasma, contrary to the concentration of mBP which is significantly lower in perfusion plasma than in foetal plasma. It is concluded that in late gestation the foetal exposure for mBP is due more to the foetal metabolic conversion of BP than to the foetal exposure of mBP produced by maternal enzymatic activity.