A comparison of engineered urinary bladder and intestinal smooth muscle for urinary bladder wall replacement in a rabbit model
A comparison of engineered urinary bladder and intestinal smooth muscle for urinary bladder wall replacement in a rabbit model
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DOI：
10.1016/j.jpedsurg.2006.08.013
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发表时间：
2006-12-01
影响因子：
2.4
通讯作者：
Lin, Jer-Nan
中科院分区：
文献类型：
--
作者：
Lai, Jin-Yao;Chang, Pei-Yeh;Lin, Jer-Nan
背景/目的：小肠是膀胱扩大术最常见的来源。肠平滑肌细胞（SMC）是否可以被工程化到膀胱组织中还知之甚少。我们在兔模型中研究了工程化膀胱和肠平滑肌细胞作为膀胱壁替代物的表型和功能特征。在最初的70%部分囊肿切除后一个月，进行了3次自动隆乳手术，包括传统的自动隆乳手术。（TA，n = 6）、使用工程化膀胱SMC的TA（TA + B，n = 6）和使用肠SMC的TA（TA + 1，n = 6）。术后第1、3、6个月随访，测量膀胱体积，并取膀胱组织行免疫细胞化学和电场刺激（EFS）检测。结果：TA + I组和TA + B组在所有时间点均表现出显著且相似的膀胱容量增加。工程化的肌肉细胞表现出典型的“收缩-舒张”反应，以超最大EFS。TA + I组和TA + B组的收缩力差异无统计学意义。结论：膀胱和小肠组织工程化平滑肌细胞在体内植入后均能保持其表型。两者都表现出类似程度的收缩EFS。这些结果表明，从2个不同器官获得的肌细胞之间没有表型或功能差异。两者都有可能被改造成正常的膀胱组织。(c)2006年爱思唯尔公司All rights reserved.
Background/Purpose: The small intestine is the most common resource for bladder augmentation. Little is known whether intestinal smooth muscle cells (SMCs) may be engineered into bladder tissue. We investigated the phenotypic and functional characteristics of engineered bladder and intestinal SMCs as bladder wall replacement in a rabbit model.Methods: One month after an initial 70% partial cystectomy, 3 autoaugmentation surgeries were performed, including traditional autoaugmentation (TA, n = 6), TA using engineered bladder SMCs (TA + B, n = 6), and TA using intestinal SMCs (TA + 1, n = 6). All were followed up by bladder volume measurement and retrieved on the first, third, and sixth month, The grafts and the native bladder wall were evaluated with immunocytochemistry and electrical field stimulation (EFS). Statistical analysis was performed using analysis of variance.Results: Both the TA + I and TA + B groups showed significant and similar bladder capacity increment in all time points. The engineered muscle cells demonstrated the typical "contraction-relaxation" response to supramaximal EFS. There were no statistical differences in both the TA + I and TA + B groups in contractility force.Conclusion: Engineered SMCs derived from urinary bladder and small intestine could retain their phenotype after implantation in vivo. Both exhibited a similar degree of contractility to EFS. These results suggest that there are no phenotypic or functional differences between muscle cells obtained from the 2 different organs. Both have the potential to be engineered into normal bladder tissues. (c) 2006 Elsevier Inc. All rights reserved.