New Bacterial Phytase through Metagenomic Prospection
New Bacterial Phytase through Metagenomic Prospection
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DOI：
10.3390/molecules23020448
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发表时间：
2018-02-01
期刊：
影响因子：
4.6
通讯作者：
Meneses, Carlos
中科院分区：
文献类型：
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作者：
Farias, Nathalya;Almeida, Isabela;Meneses, Carlos
非培养微生物来源的碱性植酸酶可将植酸水解为磷酸化程度较低的肌醇和无机磷酸盐，在农业添加剂方面具有很大的应用潜力。宏基因组学的发展源于一个不可避免的证据，即尚未培养的微生物代表了地球上大多数环境中的绝大多数生物体。本研究利用宏基因组学技术从红米作物残体和蓖麻饼粕中克隆了一个植酸酶基因。该基因与其最接近的已发表对应物之间的氨基酸同一性低于60%。该植酸酶被命名为PhyRC 001，并进行了生化表征。该重组蛋白对植酸钠具有活性，表明PhyRC 001是一种水解酶。酶活性在pH为7.0和温度为35 ℃时最佳。β-螺旋桨植酸酶是目前唯一一种在中性pH条件下具有较高活性的植酸酶，具有作为饲料添加剂的巨大潜力，因此，深入研究β-螺旋桨植酸酶的作用机制对生物技术的发展具有重要意义。
Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 degrees C. beta-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for beta-propeller phytase functions could be of great benefit to biotechnology.