Quantitative proteomic analysis of the budding yeast cell cycle using acid-cleavable isotope-coded affinity tag reagents
Quantitative proteomic analysis of the budding yeast cell cycle using acid-cleavable isotope-coded affinity tag reagents
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DOI：
10.1002/pmic.200600159
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发表时间：
2006-12-01
期刊：
影响因子：
3.4
通讯作者：
Aebersold, Ruedi
中科院分区：
文献类型：
--
作者：
Flory, Mark R.;Lee, Hookeun;Aebersold, Ruedi
蛋白质是几乎所有生物功能的直接效应物，其定量分析无疑将补充mRNA测量技术。通过使用同位素编码的亲和标签试剂和用于LC-MS/MS数据的高通量分析的软件工具进行稳定同位素标记，现在可以对细胞周期进行系统的蛋白质组学测量。我们在这里提供了第一个这样的研究实现定量，全球蛋白质组学测量的时间过程中的基因表达实验在一个模型真核生物，芽殖酵母酿酒酵母，在细胞周期。我们从所有预测的ORF中抽取了48%的样本，并以可排序的矩阵向社区提供了数据，包括鉴定、定量和确定性的统计测量。我们没有检测到显着的一致性，在我们的蛋白质组测量和微阵列测量收集类似处理的酵母培养物之间的时间过程中测试的动态系统。因此，我们的蛋白质组学数据集为真核生物基因表达的研究提供了必要的补充手段，为S.酿酒酵母蛋白质，并将使进一步发展的技术，全球蛋白质组学分析的高等真核生物。
Quantitative profiling of proteins, the direct effectors of nearly all biological functions, will undoubtedly complement technologies for the measurement of mRNA. Systematic proteomic measurement of the cell cycle is now possible by using stable isotopic labeling with isotope-coded affinity tag reagents and software tools for high-throughput analysis of LC-MS/MS data. We provide here the first such study achieving quantitative, global proteomic measurement of a time-course gene expression experiment in a model eukaryote, the budding yeast Saccharomyces cerevisiae, during the cell cycle. We sampled 48% of all predicted ORFs, and provide the data, including identifications, quantitations, and statistical measures of certainty, to the community in a sortable matrix. We do not detect significant concordance in the dynamics of the system over the time-course tested between our proteomic measurements and microarray measures collected from similarly treated yeast cultures. Our proteomic dataset therefore provides a necessary and complementary measure of eukaryotic gene expression, establishes a rich database for the functional analysis of S. cerevisiae proteins, and will enable further development of technologies for global proteomic analysis of higher eukaryotes.