Permeability of Brain Tumor Vessels Induced by Uniform or Spatially Microfractionated Synchrotron Radiation Therapies
Permeability of Brain Tumor Vessels Induced by Uniform or Spatially Microfractionated Synchrotron Radiation Therapies
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DOI：
10.1016/j.ijrobp.2017.03.025
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发表时间：
2017-08-01
影响因子：
7
通讯作者：
Serduc, Raphael
中科院分区：
文献类型：
--
作者：
Bouchet, Audrey;Potez, Marine;Serduc, Raphael
目的：比较同步辐射微束照射对血脑屏障通透性的影响方法和材料：将患有恶性颅内F98胶质瘤的雄性大鼠随机分为3组：未治疗组、暴露于MRT的组、暴露于MRT的组、暴露于MRT的组、暴露于MRT的组和暴露于MRT的组。（峰值和谷值剂量：分别为241和10.5戈伊），或暴露于以相当剂量（即，相当于MRT谷值剂量）递送的宽束辐照（BB）;两者均由2个阵列施加，与肿瘤区域正交相交。在治疗开始前1天（T-1）和治疗开始后1、2、7和14天，通过磁共振成像在体内监测血管通透性。为了确定是否生理参数影响血管通透性，我们评估了血管完整性在肿瘤区域与不同值的脑血流量，血容量，水肿，和组织oxygenation.Results：微束放射治疗不修改正常脑组织的血管通透性。微束放射治疗诱导的肿瘤血管通透性增加从T-2开始检测，在照射后T-7达到最大值，而BB仅在T7增加血管通透性。在此阶段，MRT在增加肿瘤血管通透性方面更有效（BB vs未治疗：+19.1%; P = 0.0467; MRT vs未治疗：+44.8%; P = 0.0001）。
Purpose: To compare the blood-brain barrier permeability changes induced by synchrotron microbeam radiation therapy (MRT, which relies on spatial fractionation of the incident x-ray beam into parallel micron-wide beams) with changes induced by a spatially uniform synchrotron x-ray radiation therapy.Methods and Materials: Male rats bearing malignant intracranial F98 gliomas were randomized into 3 groups: untreated, exposed to MRT (peak and valley dose: 241 and 10.5 Gy, respectively), or exposed to broad beam irradiation (BB) delivered at comparable doses (ie, equivalent to MRT valley dose); both applied by 2 arrays, intersecting orthogonally the tumor region. Vessel permeability was monitored in vivo by magnetic resonance imaging 1 day before (T-1) and 1, 2, 7, and 14 days after treatment start. To determine whether physiologic parameters influence vascular permeability, we evaluated vessel integrity in the tumor area with different values for cerebral blood flow, blood volume, edema, and tissue oxygenation.Results: Microbeam radiation therapy does not modify the vascular permeability of normal brain tissue. Microbeam radiation therapyeinduced increase of tumor vascular permeability was detectable from T-2 with a maximum at T-7 after exposure, whereas BB enhanced vessel permeability only at T7. At this stage MRT was more efficient at increasing tumor vessel permeability (BB vs untreated: +19.1%; P = .0467; MRT vs untreated: +44.8%; P