Force Spectroscopy as a Tool to Investigate the Properties of Supported Lipid Membranes
Force Spectroscopy as a Tool to Investigate the Properties of Supported Lipid Membranes
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DOI：
10.1002/jemt.20834
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发表时间：
2010-10-01
影响因子：
2.5
通讯作者：
Dante, S.
中科院分区：
文献类型：
--
作者：
Canale, C.;Jacono, M.;Dante, S.
固体支持的脂质双层（SLB）被广泛用作各种光谱和生物物理方法中研究细胞膜的模型。然而，众所周知的是，与固体基质如云母或硅的相互作用影响膜的性质。在这篇文章中，我们采用原子力显微镜（AFM）的力谱模式（FS）研究的局部力学性能的脂质膜支撑在云母和聚合物垫。磷脂单层囊泡融合形成双层脂质结构。聚合物载体通过带电聚电解质的自组装产生。力谱提供了关于穿透力、穿透深度和样品粘附性的信息。提出了一种处理高分辨率力映射的批处理算法。将双层向下粘附到支持物的穿透力和粘附力作为膜电荷的函数进行测量。从云母上的SLB和从聚合物垫上的双层获得的数据的比较提供了直接的证据的影响的基板上的膜的局部机械性能。作为一个主要的结果，聚合物垫上的膜的屈服力分布是双峰的，相比之下，在云母上得到的单峰分布。Microsc. Res. Tech. 73：965-972，2010. (C)2010年威利-利斯。Inc.
Solid supported lipid bilayers (SLB) are extensively used as a model for the investigation of cell membranes in a variety of spectroscopic and biophysical methods. It is nevertheless well known that the interaction with the solid substrate, such as mica or silicon, influences the properties of the membranes. In this article we have employed atomic force microscopy (AFM) in force spectroscopy mode (FS) to investigate the local mechanical properties of lipid membranes supported on mica and on polymer cushion. The lipid double layers were obtained by fusion of unilamellar vesicle of phospholipids. The polymer support was created by self-assembly of charged polyelectrolytes. Force spectroscopy provided information about the breakthrough force, the breakthrough depth, and the sample adhesion. A batch analysis algorithm to process high-resolution force mapping was developed. The breakthrough force to indent the bilayers down to the support and the adhesion force were measured as a function of the membrane charge. The comparison of the data obtained from SLB on mica and from bilayers on polymer cushion provides direct evidence about the influence of the substrate on the local mechanic properties of the membrane. As a major result, the yield force distribution of membranes on polymer cushion was bimodal, compared to the unimodal distribution obtained on mica. Microsc. Res. Tech. 73:965-972, 2010. (C) 2010 Wiley-Liss. Inc.